Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences (SHUATS), Prayagraj, India.
Environ Toxicol. 2021 Apr;36(4):572-585. doi: 10.1002/tox.23062. Epub 2020 Nov 28.
Current work was designed to explore the effect of ZnO nanoparticles (ZnONP) biofabricated by using Trianthema portulacastrum (TP) leaves extract on mice brain hippocampus. ZnO nanoparticles of TP leaves (ZnOTP) were synthesized by co-precipitation method and further characterized by using various techniques such as UV-Vis spectrophotometer, Field Emission Scanning Electron Microscopy (FESEM), Fourier Transform Infrared (FTIR), and Energy Dispersive X-ray (EDX). ZnOTP were evaluated for in vitro antioxidant activity, in vivo behavior models (for assessment of cognitive ability), acetylcholinesterase (AChE) activity along with other neurotransmitters content determination, estimation of various oxidative stress parameters and analysis of zinc content in the brain as well as plasma. Histopathological evaluation of the brain hippocampus of each group was performed to corroborate the statistical results. Spherical ZnOTP of 10 to 20 nm size embedded with different phytoconstituents of TP was confirmed. Results of our study revealed a significant memory deficit in mice treated with ZnOTP. Neuronal degeneration was also observed via a significant increase in AChE activity and oxidative stress levels in the brain of mice administered with ZnOTP. Exposure of ZnOTP was also found responsible for modulation of neurotransmission in hippocampus area. Further, ZnOTP disturbed the zinc homeostasis in hippocampus via elevation of zinc content in brain as well as plasma. Histopathology of hippocampus supported the damaging impact of ZnOTP by an increase in vacuolated cytoplasm and focal gliosis in groups treated with ZnOTP. Results demonstrated the neurotoxic effect of ZnOTP on brain hippocampus via cognitive impairment by alteration of neurotransmitter level, zinc content and oxidative stress.
当前的工作旨在探索使用 Trianthema portulacastrum（TP）叶提取物生物制造的氧化锌纳米粒子（ZnONP）对小鼠大脑海马体的影响。通过共沉淀法合成了 TP 叶的氧化锌纳米粒子（ZnOTP），并使用各种技术进一步进行了表征，如紫外可见分光光度计、场发射扫描电子显微镜（FESEM）、傅里叶变换红外（FTIR）和能量色散 X 射线（EDX）。评估了 ZnOTP 的体外抗氧化活性、体内行为模型（用于评估认知能力）、乙酰胆碱酯酶（AChE）活性以及其他神经递质含量的测定、各种氧化应激参数的估算以及大脑和血浆中锌含量的分析。对每组大脑海马体进行了组织病理学评估，以证实统计结果。证实了 10 至 20nm 大小的 ZnOTP 嵌入了 TP 的不同植物成分。我们的研究结果表明，用 ZnOTP 处理的小鼠存在明显的记忆缺陷。用 ZnOTP 处理的小鼠大脑中 AChE 活性和氧化应激水平的显著增加也观察到神经元变性。还发现 ZnOTP 的暴露负责调节海马区的神经递质传递。此外，ZnOTP 通过提高大脑和血浆中锌的含量来破坏海马体中的锌稳态。海马体的组织病理学支持了 ZnOTP 通过增加用 ZnOTP 处理的组中的空泡化细胞质和局灶性神经胶质增生对海马体的破坏性影响。结果表明，ZnOTP 通过改变神经递质水平、锌含量和氧化应激对大脑海马体产生神经毒性作用，导致认知障碍。
