青蒿素对人乳腺癌干细胞中 Hippo 信号通路组件（LATS1、LATS2 和 YAP）表达的影响。
Effects of Quinacrine on Expression of Hippo signaling Pathway Components (LATS1, LATS2, and YAP) in Human Breast Cancer Stem Cells.
机构信息
Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetic, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Asian Pac J Cancer Prev. 2020 Nov 1;21(11):3171-3176. doi: 10.31557/APJCP.2020.21.11.3171.
OBJECTIVE
The Hippo signaling pathway has important role in the pathogenesis of some tumors. Breast cancer is the most prevalent cancer among females in the world. In recent years, various articles referred to inhibiting effect of quinacrine, a derivative of 9-aminoacridine, on the growth of several types of cancer cells. In this study, we evaluated the effect of quinacrine on expression of LATS1, LATS2, and YAP genes of the Hippo signaling pathway and YAP level in human breast cancer stem cells (MDA-MB 231 cell line). This cell line of breast cancer expresses the triple negative characteristics.
METHODS
MDA-MB 231 cells was treated with 0.5 µM of quinacrine for 3 days. The dose was selected using MTT assays. The expression of genes was quantified by Real-time PCR. The protein expression was performed by Western blotting. Significance of observations were checked by means of Mann-Whitney test using p.
目的
Hippo 信号通路在一些肿瘤的发病机制中具有重要作用。乳腺癌是全世界女性中最常见的癌症。近年来，有各种文章提到了 9-氨基吖啶的衍生物奎纳克林对几种类型癌细胞生长的抑制作用。在这项研究中，我们评估了奎纳克林对 Hippo 信号通路的 LATS1、LATS2 和 YAP 基因表达以及人乳腺癌干细胞（MDA-MB 231 细胞系）中 YAP 水平的影响。该乳腺癌细胞系表达三阴性特征。
方法
用 0.5µM 的奎纳克林处理 MDA-MB 231 细胞 3 天。采用 MTT 试验选择剂量。通过 Real-time PCR 定量基因表达。通过 Western blotting 进行蛋白表达。使用 p 值的 Mann-Whitney 检验检查观察结果的显著性。
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