Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Federal University of Santa Maria, Santa Maria, RS, Brazil; University Hospital, Santa Maria, RS, Brazil.
Anal Biochem. 2021 Feb 1;614:114047. doi: 10.1016/j.ab.2020.114047. Epub 2020 Nov 26.
Urinary albumin is one of the main markers used in clinical practice to assess kidney damage. It is usually measured in laboratories through immunological assays, but these assays may not detect molecules with conformational changes, such as carbamylated albumin/proteins. Therefore, this study aimed to investigate the impact of albumin carbamylation on the measurement of albuminuria by an immunoturbidimetric assay. The addition of the carbamylating agent to PBS buffer and urine pool promoted a lower quantification of albumin measured by the immunoturbidimetric method, indicating that this process may be responsible for an underestimation of the results in clinical practice.
尿白蛋白是临床实践中用于评估肾脏损伤的主要标志物之一。它通常通过免疫学检测在实验室中进行测量，但这些检测方法可能无法检测到具有构象变化的分子，如碳化白蛋白/蛋白质。因此，本研究旨在探讨白蛋白碳化对免疫比浊法检测白蛋白尿的影响。在 PBS 缓冲液和尿池中加入碳化剂后，用免疫比浊法测量的白蛋白量明显减少，表明这一过程可能导致临床实践中对结果的低估。
