Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, PR China.
Shandong Key Laboratory of Animal Microecological Preparation, Shandong Baolai-Leelai Bio-Tech Co., Ltd, No.28th, Chuangye Street, Taishan District, Tai'an, 271000, PR China.
Fish Shellfish Immunol. 2021 Jan;108:32-41. doi: 10.1016/j.fsi.2020.11.025. Epub 2020 Nov 26.
Intelectin (ITLN) is a type of glycan-binding lectin involved in many physiological processes and some human diseases. Here we report a common carp intelectin (cITLN). Like other orthologs, cITLN also contains a conserved fibrinogen-related domain (FReD) and a unique intelectin domain, expresses in all the tissues tested with the highest level in the hindgut, and responds to bacterial challenge in the acute phase. We also expressed cITLN in Escherichia coli (E. coli) system, and the purified recombinant cITLN could neither affect the surface of bacteria nor inhibit the growth of bacteria, but it can agglutinate both gram-positive and gram-negative bacteria in a calcium-dependent manner. The cITLN's ability of agglutination of gram-positive bacteria is stronger than that of gram-negative bacteria. This is probably because recombinant cITLN could binding peptidoglycan (PGN) with a higher degree to lipopolysaccharide (LPS). Our results of cITLN provided new insight into the function of intelectin in the intestinal mucosal immunity.
凝集素（ITLN）是一种参与多种生理过程和一些人类疾病的糖结合凝集素。本文报道了一种鲤鱼凝集素（cITLN）。与其他同源物一样，cITLN 也含有一个保守的纤维蛋白原相关结构域（FReD）和一个独特的凝集素结构域，在所有检测的组织中均有表达，在后肠中的表达水平最高，并在急性期对细菌挑战产生反应。我们还在大肠杆菌（E. coli）系统中表达了 cITLN，纯化的重组 cITLN 既不能影响细菌表面，也不能抑制细菌生长，但它可以依赖于钙来凝集革兰氏阳性菌和革兰氏阴性菌。cITLN 凝集革兰氏阳性菌的能力强于革兰氏阴性菌。这可能是因为重组 cITLN 可以与肽聚糖（PGN）结合，与脂多糖（LPS）结合的程度更高。我们对 cITLN 的研究结果为凝集素在肠道黏膜免疫中的功能提供了新的见解。
