Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
Kyushu Okinawa Agriculture Research Center, National Agriculture and Food Research Organization (NARO), Koshi, Japan.
Pest Manag Sci. 2021 Apr;77(4):1786-1795. doi: 10.1002/ps.6200. Epub 2020 Dec 14.
The brown planthopper, Nilaparvata lugens (Stål), is one of the most notorious pests of rice throughout Asia. The brown planthopper has developed high resistance to imidacloprid, a member of neonicotinoid insecticides. Several genes and mutations conferring imidacloprid resistance in N. lugens, especially in eastern and southeastern Asia populations, have been reported. Thus, the key mechanisms of imidacloprid resistance need to be examined.
RNA-seq analyses revealed that only one cytochrome P450 monooxygenase gene, CYP6ER1, was commonly upregulated in the five resistant strains tested. Sequences of CYP6ER1, which were highly expressed in the imidacloprid-resistant strains, contained a three-nucleotide deletion in the coding region, and amino acid substitutions and deletion, compared to that in an imidacloprid-susceptible strain. RNAi-mediated gene knockdown of CYP6ER1 increased imidacloprid susceptibility in a resistant strain. Further, we established two simple and convenient PCR-based molecular diagnostic methods to detect the CYP6ER1 locus with the three-nucleotide deletion. Using these methods, the resistance of F progenies derived from the crosses of F siblings from susceptible and resistant parents was analyzed, showing that the imidacloprid resistance had a relationship to the CYP6ER1 locus with the three-nucleotide deletion.
The overexpression of a variant CYP6ER1 with amino acid substitutions and deletion was involved in imidacloprid resistance in N. lugens. Based on these findings, molecular diagnostic methods have been developed and are promising tools for monitoring imidacloprid resistance in paddy fields. © 2020 Society of Chemical Industry.
褐飞虱，Nilaparvata lugens（Stål），是亚洲各地水稻的一种最臭名昭著的害虫。褐飞虱对吡虫啉产生了很高的抗性，吡虫啉是新烟碱类杀虫剂的一种。已报道几种在 N. lugens 中赋予对吡虫啉抗性的基因和突变，特别是在东亚和东南亚种群中。因此，需要检查吡虫啉抗性的关键机制。
RNA-seq 分析表明，在所测试的五个抗性品系中，仅有一个细胞色素 P450 单加氧酶基因 CYP6ER1 普遍上调。在对吡虫啉具有抗性的品系中高度表达的 CYP6ER1 序列，在编码区中包含三个核苷酸缺失，与对吡虫啉敏感的品系相比，存在氨基酸取代和缺失。CYP6ER1 的 RNAi 介导基因敲低增加了抗性品系对吡虫啉的敏感性。此外，我们建立了两种简单方便的基于 PCR 的分子诊断方法，以检测具有三个核苷酸缺失的 CYP6ER1 基因座。使用这些方法分析了来自对吡虫啉敏感和抗性亲本的 F 同胞杂交的 F 后代的抗性，表明吡虫啉抗性与具有三个核苷酸缺失的 CYP6ER1 基因座有关。
具有氨基酸取代和缺失的变异 CYP6ER1 的过表达与 N. lugens 对吡虫啉的抗性有关。基于这些发现，已经开发了分子诊断方法，并且是监测稻田中吡虫啉抗性的有前途的工具。 © 2020 化学工业协会。
