小鼠葡萄糖磷酸异构酶（GPI）同工酶的免疫化学鉴别

Immunochemical differentiation of glucose phosphate isomerase (GPI) allozymes of the mouse.

作者信息

Conrad U, Walter G, Helbing D, Manteuffel R, Schöneich J

机构信息

Zentralinstitut für Genetik und Kulturpflanzenforschung der AdW der DDR, Gatersleben, German Democratic Republic.

出版信息

Biochem Genet. 1987 Oct;25(9-10):739-54. doi: 10.1007/BF00556216.

DOI:10.1007/BF00556216

PMID:3325033

Abstract

Polyclonal xenoantisera against mouse GPI-1B and GPI-1C were produced in rabbits and analyzed for their ability to recognize allozyme-specific determinants. These studies showed a high degree of serological similarity among the three allozymes of mouse glucose phosphate isomerase (GPI). However, GPI-1B and GPI-1C could be differentiated from GPI-1A as well as GPI-1A and GPI-1B from GPI-1C using quantitative solid-phase immunobinding assays. In addition, polyclonal and monoclonal alloantibodies specific for GPI-1C were produced in BALB/c (Gpi-1a/Gpi-1a) mice. As indicated by immunoblotting data, the allozyme specificity of rabbit antisera and monoclonal alloantibodies against GPI-1C is dependent on the native structure of that allozyme.

摘要

用兔子制备了针对小鼠GPI-1B和GPI-1C的多克隆异种抗血清，并分析了它们识别同工酶特异性决定簇的能力。这些研究表明，小鼠葡萄糖磷酸异构酶（GPI）的三种同工酶之间存在高度的血清学相似性。然而，使用定量固相免疫结合试验，可以将GPI-1B和GPI-1C与GPI-1A区分开来，也可以将GPI-1A和GPI-1B与GPI-1C区分开来。此外，在BALB/c（Gpi-1a/Gpi-1a）小鼠中产生了针对GPI-1C的多克隆和单克隆同种抗体。免疫印迹数据表明，兔抗血清和针对GPI-1C的单克隆同种抗体的同工酶特异性取决于该同工酶的天然结构。