Department of Medical Microbiology, Faculty of Medicine, Alanya Alaaddin Keykubat University, Turkey.
National Molecular Microbiology Reference Laboratory, Public Health General Directorate, Ministry of Health, Sıhhiye, Turkey.
Jpn J Infect Dis. 2021 May 24;74(3):200-208. doi: 10.7883/yoken.JJID.2020.478. Epub 2020 Nov 30.
We aimed to investigate the clonal relationships, common sequence types, and carbapenemase genes in 177 non-repetitive blood culture isolates of Acinetobacter baumannii collected from patients at three university hospitals in Turkey in 2016. Molecular epidemiological characteristics of the isolates were examined using pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) (Pasteur scheme-cpn60, fusA, gltA, pyrG, recA, rplB, and rpoB). Multiplex PCR was used to investigate the carbapenemase genes, including bla, bla, bla, bla, bla, bla, and bla. PFGE genotyping yielded 92 pulsotypes with a clustering ratio of 69.7%. As per a ≥85% similarity coefficient, 159 (90.9%) isolates were found to be clonally related. The bla and bla genes were identified in 100% and 28.2% of the isolates, respectively. The bla gene was identified in two isolates. The MLST analysis included 54 isolates with different pulsotypes, and 29 sequence types (STs). Most of the isolates (n = 36) belonged to the clonal complex (CC)2, one isolate belonged to CC1, and one isolate belonged to CC164. Sixteen new STs (ST1235-ST1250) were identified. Identifying both global ST2 and a large number of new STs, revealed high genetic diversity in A. baumannii isolates in the study population.
本研究旨在调查 2016 年从土耳其三所大学医院的患者血液培养分离的 177 株非重复鲍曼不动杆菌的克隆关系、常见序列型和碳青霉烯酶基因。采用脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）（巴斯德方案-cpn60、fusA、gltA、pyrG、recA、rplB 和 rpoB）对分离株的分子流行病学特征进行了检测。采用多重 PCR 检测碳青霉烯酶基因，包括 bla、bla、bla、bla、bla、bla 和 bla。PFGE 基因分型产生了 92 个脉冲型，聚类率为 69.7%。根据≥85%相似系数，发现 159 株（90.9%）分离株具有克隆相关性。100%和 28.2%的分离株分别携带 bla 和 bla 基因。在两个分离株中检测到 bla 基因。MLST 分析包括 54 株具有不同脉冲型的分离株和 29 种序列型（ST）。大多数分离株（n=36）属于克隆复合体（CC）2，一株属于 CC1，一株属于 CC164。鉴定出 16 种新的 ST（ST1235-ST1250）。鉴定出全球 ST2 和大量新的 ST，表明研究人群中鲍曼不动杆菌分离株具有高度遗传多样性。
