Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Int J Mol Sci. 2020 Nov 24;21(23):8886. doi: 10.3390/ijms21238886.
The prevalence of gastric (Hp) infection is ~50% of the world population. However, how Hp infection influences inflammatory bowel disease in humans is not fully defined. In this study, we examined whether co-infection with Hp influenced (Hh)-induced intestinal pathology in mice. mice of both sexes were infected with Hh, of which a subgroup was followed by infection with Hp two weeks later. Co-infected males, but not females, had significantly higher total colitis index scores in the colon at both 10 and 21 weeks post-Hh infection (WPI) and developed more severe dysplasia at 21 WPI compared with mono-Hh males. There were no significant differences in colonization levels of gastric Hp and colonic Hh between sexes or time-points. In addition, mRNA levels of colonic , , , , , , and , which play important roles in the development and function of proinflammatory innate lymphoid cell groups 1 and 3, were significantly up-regulated in the dually infected males compared with mono-Hh males at 21 WPI. These data suggest that concomitant Hp infection enhances the inflammatory responses in the colon of-Hh-infected males, which results in more severe colitis and dysplasia.
全球约有 50%的人口感染幽门螺杆菌（Hp）。然而，Hp 感染如何影响人类炎症性肠病尚不完全清楚。在这项研究中，我们研究了 Hp 合并感染是否会影响 Hh 诱导的小鼠肠道病理。雌雄小鼠均感染 Hh，其中一部分在两周后感染 Hp。与单感染 Hh 的雄性小鼠相比，合并感染的雄性小鼠在感染 Hh 后 10 和 21 周（WPI）的结肠总结肠炎指数评分显著更高，并在 21 WPI 时发生更严重的异型增生，但雌雄小鼠的胃 Hp 和结肠 Hh 的定植水平在各时间点均无显著差异。此外，与单感染 Hh 的雄性小鼠相比，合并感染的雄性小鼠在 21 WPI 时结肠中与固有淋巴样细胞群 1 和 3 发育和功能相关的、 、 、 、 、 和 的 mRNA 水平显著上调。这些数据表明，Hp 合并感染增强了-Hh 感染雄性小鼠结肠的炎症反应，导致更严重的结肠炎和异型增生。
