Jonson Gilda B, Matres Jerlie M, Ong Socheath, Tanaka Toshiharu, Choi Il-Ryong, Chiba Sotaro
Rice Breeding Platform, International Rice Research Institute, Los Baños, Laguna 4031, Philippines.
Department of Crop Protection, Faculty of Agronomy, Royal University of Agriculture, Ministry of Agriculture, Forestry and Fisheries, Chamkar Duang, Dangkor District, Phnom Penh 370, Cambodia.
Pathogens. 2020 Nov 26;9(12):990. doi: 10.3390/pathogens9120990.
Rice orange leaf phytoplasma (ROLP) belongs to the " Phytoplasma asteris" 16SrI-B subgroup, which is solely transmitted by the zigzag-striped leafhopper ( Motchulsky) and the green leafhopper ( Uhler) (). Recently, rice plants showing orange leaf discoloration have become ubiquitous in several paddies of two provinces in the Philippines. In total of 98 symptomatic rice plants, 82% (Laguna) and 95% (Mindanao) were ROLP-positive by nested PCR detection. These plants showed more varying symptoms than previously reported. The vector insect was scarcely present but green paddy leafhopper, Distant (), was commonly observed in the paddies, thus the ability of to transmit ROLP was thoroughly investigated. Newly emerged adult , which fed on ROLD-source rice plants, were used to inoculate a susceptible rice seedling and was serially transferred into a new healthy seedling. Resultant positive transmission rates varied from 5.1% to 17.8%. The transmission ability of the insects was generally decreased over time. These findings suggest that is an alternative vector of ROLP in the Philippines. Altogether, this study highlighted the increasing importance of ROLD-reemergence in Southeast and East Asia and proved the need for careful management of this alternative vector insect.
水稻橙叶植原体（ROLP）属于“紫菀植原体”16SrI - B亚组，仅由锯齿叶蝉（Motchulsky）和绿叶蝉（Uhler）传播（）。最近，在菲律宾两个省的几块稻田中，出现橙叶变色的水稻植株变得很普遍。在总共98株有症状的水稻植株中，通过巢式PCR检测，82%（拉古纳省）和95%（棉兰老岛）的植株ROLP呈阳性。这些植株表现出比以前报道的更多样化的症状。传病昆虫很少出现，但在稻田中常见到绿色稻叶蝉（Distant）（），因此对其传播ROLP的能力进行了全面研究。以感染ROLD的水稻植株为食的新羽化的成虫，用于接种易感水稻幼苗，并依次转移到新的健康幼苗上。产生的阳性传播率在5.1%至17.8%之间。随着时间的推移，昆虫的传播能力总体上有所下降。这些发现表明，在菲律宾，绿色稻叶蝉是ROLP的替代传病媒介。总之，本研究突出了ROLD在东南亚和东亚再度出现的重要性日益增加，并证明了对这种替代传病昆虫进行谨慎管理的必要性。
