Barale R, Migliore L, Zucconi D, Ciranni R, Casini D, Carducci A, Loprieno N
Dipartimento di Scienze dell'Ambiente e del Territorio, Università di Pisa, Italy.
Mutagenesis. 1987 May;2(3):173-7. doi: 10.1093/mutage/2.3.173.
Cyclophosphamide (CP)-induced micronuclei were evaluated in females of two strains of mice (C57BL and CD1), in F1 hybrid females and in fetuses (day 13 of gestation) obtained from different crosses. F1 adult hybrids from a cross between the strain with a high level of induced micronuclei (C57BL) and the strain with a low response (CD1) exhibited micronuclei values closer to the latter. CD1/CD1 fetuses showed a higher susceptibility than C57BL/C57BL ones. Heterozygous fetuses from reciprocal crosses, whatever the maternal genotype, showed the same sensitivity, which is very close to that of C57BL/C57BL fetuses. Phenobarbital (PB) pre-treatment modified the mutagenic response to CP depending on the genotype of the treated animal. These results demonstrate that the response to a pro-mutagen requiring metabolic activation depends to a large extent on the genetic background of the target animals, and that mother-fetus interactions in transplacental mutagenesis seem to depend more on the fetal than on the maternal genotype.
在两种品系的雌性小鼠（C57BL和CD1）、F1杂交雌性小鼠以及不同杂交组合获得的胎儿（妊娠第13天）中评估环磷酰胺（CP）诱导的微核。来自诱导微核水平高的品系（C57BL）与反应低的品系（CD1）杂交的F1成年杂种小鼠的微核值更接近后者。CD1/CD1胎儿比C57BL/C57BL胎儿表现出更高的易感性。无论母本基因型如何，正反交的杂合子胎儿表现出相同的敏感性，这与C57BL/C57BL胎儿非常接近。苯巴比妥（PB）预处理根据受试动物的基因型改变了对CP的诱变反应。这些结果表明，对需要代谢活化的前诱变剂的反应在很大程度上取决于靶动物的遗传背景，并且经胎盘诱变中的母胎相互作用似乎更多地取决于胎儿基因型而非母本基因型。
