Xu Na, Chu Jun, Dong Rongrong, Lu Fengjuan, Zhang Xinfeng, Wang Min, Shen Ying, Xie Zhongwen, Ho Chi-Tang, Yang Chung S, Wang Yijun, Wan Xiaochun
State Key Laboratory of Tea Plant Biology and Utilization, School of Tea & Food Science, Anhui Agricultural University, Hefei, Anhui, 230036, P. R. China.
International Joint Laboratory on Tea Chemistry and Health Effects of Ministry of Education, Anhui Agricultural University, Hefei, Anhui, 230036, P. R. China.
Mol Nutr Food Res. 2021 Jan;65(2):e2000864. doi: 10.1002/mnfr.202000864. Epub 2021 Jan 7.
Large-leaf yellow tea (YT) exhibits interesting beneficial metabolic effects in previous studies. Here, the authors elucidated the actions of YT on thermogenesis, energy metabolism, and adipocyte metabolic conversion.
Five-week-old male C57BL/6 mice are fed low-fat diet, high-fat diet (HFD), and HFD supplemented with 0.5% or 2.5% YT. After treatment for 10 or 14 weeks, YT enhances energy expenditure, O consumption and CO production. YT strongly boosts thermogenic program in brown adipose tissue (BAT) and subcutaneous adipose tissue (SAT), while only weakly in epididymal adipose tissue (EAT). These are accompanied by higher body temperature, increased mitochondrial copy numbers, and upregulation of thermogenic genes (Ucp1, Pgc1α, etc.) and proteins. The classic brown adipocyte markers (Eva1, Zic1) are induced only in BAT, while beige adipocyte markers (Tbx1, Tmem26) are boosted only in SAT. Furthermore, subcutaneous-originated preadipocytes are induced by YT in vitro to differentiate to brown-like adipocytes - a browning effect.
Dietary YT induces adaptive thermogenesis through increasing mitochondrial biogenesis in EAT, inducing beigeing in SAT and enhancing browning in the BAT.
在先前的研究中，大叶黄茶（YT）展现出有趣的有益代谢效应。在此，作者阐明了YT对产热、能量代谢和脂肪细胞代谢转化的作用。
给5周龄雄性C57BL/6小鼠喂食低脂饮食、高脂饮食（HFD）以及添加了0.5%或2.5%YT的HFD。处理10周或14周后，YT可提高能量消耗、氧气消耗和二氧化碳产生。YT强烈促进棕色脂肪组织（BAT）和皮下脂肪组织（SAT）中的产热程序，而对附睾脂肪组织（EAT）的促进作用较弱。这些伴随着体温升高、线粒体拷贝数增加以及产热基因（Ucp1、Pgc1α等）和蛋白质的上调。经典的棕色脂肪细胞标志物（Eva1、Zic1）仅在BAT中被诱导，而米色脂肪细胞标志物（Tbx1、Tmem26）仅在SAT中增强。此外，YT在体外可诱导皮下来源的前脂肪细胞分化为棕色样脂肪细胞——一种褐变效应。
饮食中的YT通过增加EAT中的线粒体生物合成、诱导SAT中的米色化以及增强BAT中的褐变来诱导适应性产热。
