玉米浆对黑曲霉产β-聚（L-苹果酸）的影响。
Effects of corn steep liquor on β-poly(L-malic acid) production in Aureobasidium melanogenum.
作者信息
Wang Genan, Shi Bingyi, Zhang Pan, Zhao Tingbin, Yin Haisong, Qiao Changsheng
机构信息
Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), Ministry of Education, Tianjin, 300457, People's Republic of China.
Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China.
出版信息
AMB Express. 2020 Dec 1;10(1):211. doi: 10.1186/s13568-020-01147-8.
β-poly(L-malic acid) (PMLA) is a water-soluble biopolymer used in medicine, food, and other industries. However, the low level of PMLA biosynthesis in microorganisms limits its further application in the biotechnological industry. In this study, corn steep liquor (CSL), which processes high nutritional value and low-cost characteristics, was selected as a growth factor to increase the PMLA production in strain, Aureobasidium melanogenum, and its metabolomics change under the CSL addition was investigated. The results indicated that, with 3 g/L CSL, PMLA production, cell growth, and yield (Y) were increased by 32.76%, 41.82%, and 47.43%, respectively. The intracellular metabolites of A. melanogenum, such as amino acids, organic acids, and key intermediates in the TCA cycle, increased after the addition of CSL, and the enrichment analysis showed that tyrosine may play a major role in the PMLA biosynthesis. The results presented in this study demonstrated that the addition of CSL would be an efficient approach to improve PMLA production.
β-聚（L-苹果酸）（PMLA）是一种用于医药、食品和其他行业的水溶性生物聚合物。然而，微生物中PMLA生物合成水平较低，限制了其在生物技术产业中的进一步应用。在本研究中，选择具有高营养价值和低成本特点的玉米浆（CSL）作为生长因子，以提高菌株黑曲霉中PMLA的产量，并研究添加CSL后其代谢组学的变化。结果表明，添加3 g/L CSL后，PMLA产量、细胞生长和产率（Y）分别提高了32.76%、41.82%和47.43%。添加CSL后，黑曲霉的细胞内代谢物，如氨基酸、有机酸和三羧酸循环中的关键中间体增加，富集分析表明酪氨酸可能在PMLA生物合成中起主要作用。本研究结果表明，添加CSL是提高PMLA产量的有效方法。
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