Laboratory of Physiology and Control of Reproduction, Veterinary School, State University of Ceará (UECE), Fortaleza, Brazil.
Laboratory of Animal Germplasm Conservation, Department of Animal Science, Federal Rural University of the Semi-Arid (UFERSA), Mossoró, Brazil.
Reprod Domest Anim. 2021 Feb;56(2):351-359. doi: 10.1111/rda.13872. Epub 2020 Dec 26.
Ovarian response of collared peccaries (Pecari tajacu), after hormonal stimulation with gonadotropin association (eCG/hCG), was accessed by both gene expression and follicular development. Thus, collared peccaries (n = 8) were treated with the dose used for sows (swine dose, SWD) or with dose adjusted for peccary's weight (allometric dose, ALD). The gene expression of receptors was evaluated for both gonadotropins (FSHR and LHCGR) and growth factors (proteins codified by TGFβR-1, BMPR1-A and BMPR2 genes) in antral follicles, cortex and corpora haemorrhagica (CH). Five days after gonadotropin injection, all females presented CH. The ovulation rate was similar (p > .05) between SWD (4.00 ± 1.17) and ALD (2.50 ± 0.43) group. The total number of follicles per animal and amounts of small (<3 mm), medium (3-5 mm) and large (>5 mm) follicles was similar among groups. However, SWD produced large follicles heavier than ALD group, as accessed by weight of follicular wall biopsies. Ovarian follicles expressed both gonadotropin and growth factor receptors at levels which are independent from gonadotropin dose. In conclusion, the two gonadotropin doses (SWD and ALD) can be used for ovarian stimulation of collared peccary. Additionally, FSH and growth factors (TGFβR-1, BMPR1-A and BMPR2) receptors are more expressed in the early follicle development, while LH receptor seems to be more important in the final of follicular growth.
经激素刺激（促性腺激素联合，eCG/hCG）后，通过基因表达和卵泡发育来评估环颈野猪（Pecari tajacu）的卵巢反应。因此，用用于母猪的剂量（猪剂量，SWD）或根据野猪体重调整的剂量（比例剂量，ALD）处理了 8 只环颈野猪。评估了两种促性腺激素（FSHR 和 LHCGR）和生长因子（TGFβR-1、BMPR1-A 和 BMPR2 基因编码的蛋白质）在窦卵泡、皮质和黄体中受体的基因表达。在促性腺激素注射后 5 天，所有雌性动物均出现黄体。排卵率在 SWD（4.00±1.17）和 ALD（2.50±0.43）组之间相似（p>.05）。每组每只动物的卵泡总数以及小卵泡（<3mm）、中卵泡（3-5mm）和大卵泡（>5mm）的数量相似。然而，SWD 产生的大卵泡比 ALD 组重，这可以通过卵泡壁活检的重量来评估。卵巢卵泡表达的促性腺激素和生长因子受体水平与促性腺激素剂量无关。总之，两种促性腺激素剂量（SWD 和 ALD）可用于环颈野猪的卵巢刺激。此外，FSH 和生长因子（TGFβR-1、BMPR1-A 和 BMPR2）受体在早期卵泡发育中表达更丰富，而 LH 受体似乎在卵泡生长的最后阶段更为重要。
