Faculty of Medicine, Dermatology Department, American University of Beirut, Beirut, Lebanon.
Faculty of Sciences, Biology Department, Lebanese University, Zahle, Lebanon.
Photodermatol Photoimmunol Photomed. 2021 May;37(3):202-213. doi: 10.1111/phpp.12635. Epub 2020 Dec 11.
We hypothesized that upon sun exposure, a sub-population of primary skin-derived mesenchymal-like cells is deleteriously affected and thus contribute to the chronic inflammatory state in autosomal recessive variegate porphyria patients. The aim of this study was to isolate and characterize the mesenchymal-like stem cells from different areas of the skin in a porphyria patient (sun exposed, SE, and sun protected, SP) and to compare them with cells from a healthy individual.
The proliferation rate and the migration ability of SE and SP cells were evaluated in the presence of an antioxidant compound, N-acetylcysteine. A co-culture of SE-damaged cells with the conditioned medium from the enriched mesenchymal cell-like SP population was performed in order to regenerate the dermal injured tissue after sun exposure in patients.
Results showed that the percentage of CD105 cells varies between 3.9% in SP and 5% in SE of the healthy individual and between 3.6% and 1.4% in SP and SE in the porphyria patient, respectively. The osteogenic differentiation potential was lower in the porphyria patient when compared to the control. Furthermore, the expression of stem cell markers was more pronounced in SE than in SP cells of both control and porphyria. The use of N-acetyl cysteine did not show any beneficial effects on porphyria SE cells. Treatment with SP-conditioned medium slightly increased the expression of stem cell markers in SE of porphyria patient.
In conclusion, the pool of mesenchymal stem-like SE cells is affected in variegate porphyria patient along with modification of their self-renewal and differentiation properties.
我们假设，在阳光暴露下，原发性皮肤衍生的间充质样细胞的亚群受到有害影响，从而导致常染色体隐性遗传斑驳性卟啉症患者的慢性炎症状态。本研究的目的是从卟啉症患者（阳光暴露，SE 和阳光保护，SP）的不同皮肤区域分离和鉴定间充质样干细胞，并将其与健康个体的细胞进行比较。
在抗氧化剂 N-乙酰半胱氨酸存在的情况下，评估 SE 和 SP 细胞的增殖率和迁移能力。为了在患者暴露于阳光后再生受损的皮肤组织，进行了 SE 受损细胞与富含间充质细胞样 SP 群体的条件培养基的共培养。
结果表明，健康个体的 SP 中 CD105 细胞的百分比在 3.9%到 5%之间，SE 中为 3.6%到 1.4%之间，而卟啉症患者的 SP 和 SE 中分别为 3.6%和 1.4%。与对照组相比，卟啉症患者的成骨分化潜力较低。此外，与 SP 细胞相比，SE 细胞中的干细胞标志物表达更为明显，无论是在对照组还是卟啉症患者中均如此。使用 N-乙酰半胱氨酸对卟啉症 SE 细胞没有显示出任何有益效果。用 SP 条件培养基处理略微增加了卟啉症患者 SE 中干细胞标志物的表达。
总之，斑驳性卟啉症患者的 SE 间充质样干细胞池受到影响，同时改变了它们的自我更新和分化特性。
