采用已验证的反相高效液相色谱-光电二极管阵列检测法对来自印度的 9 种叶下珠属植物中的 4 种木脂素（叶下珠素、隐叶下珠素、新紫檀芪和新紫檀素）进行比较分析。

Comparative Profiling of Four Lignans (Phyllanthin, Hypophyllanthin, Nirtetralin, and Niranthin) in Nine Phyllanthus Species from India Using a Validated Reversed Phase HPLC-PDA Detection Method.

机构信息

ICAR-Directorate of Medicinal and Aromatic Plants Research, Anand, 387310, Gujarat, India.

Department of Botany, Faculty of Science, The M.S. University of Baroda, Vadodara, 390002, Gujarat, India.

出版信息

J AOAC Int. 2021 May 21;104(2):485-497. doi: 10.1093/jaoacint/qsaa133.

DOI:10.1093/jaoacint/qsaa133

PMID:33259591

Abstract

BACKGROUND

Phyllanthus species exhibit a wide range of in vitro and in vivo pharmacological activities; however, little is known about the compounds present in the extracts that are responsible for such actions.

OBJECTIVE

Development and validation of a simple reversed phase HPLC-PDA method for profiling of phyllanthin, hypophyllanthin, nirtetralin, and niranthin in extracts of Phyllanthus species was carried out.

METHODS

Separation was achieved using an XBridge column® (150 × 4.6 mm, 5.0 µm id) in an isocratic elution mode with mobile phase comprising of a mixture of acetonitrile and water with TFA (0.05%, v/v, pH = 2.15) at ambient temperature with a flow rate of 1 mL/min.

RESULTS

Phyllanthin, hypophyllanthin, nirtetralin, and niranthin were eluted at mean retention times of 10.47, 11.10, 13.67, and 14.53 min, respectively. LOD and LOQ for all four analytes were 0.75 and 3.00 μg/mL, respectively. RSDr values for intraday and interday precision for phyllanthin, hypophyllanthin, nirtetralin, and niranthin were 0.38-1.32 and 0.45-1.77%; 0.22-3.69 and 0.24-3.04%, 0.73-2.37 and 0.09-0.31%, and 1.56-2.77 and 0.12-0.68%, respectively.

CONCLUSIONS

The developed and validated HPLC-PDA method was applied for identification and quantification of phyllanthin, hypophyllanthin, nirtetralin, and niranthin in extracts of different plant parts of selected Phyllanthus species. The outcome of the present investigation could be useful for selection of best species to promote its commercial cultivation and suitable extraction solvent for preparation of lignan-enriched fractions. This HPLC-PDA method could be useful for quality control of herbal formulations containing plants from Phyllanthus species.

摘要

背景

叶下珠属植物表现出广泛的体外和体内药理活性；然而，对于负责这些作用的提取物中的化合物知之甚少。

目的

开发并验证了一种简单的反相高效液相色谱-光电二极管阵列法，用于分析叶下珠属植物提取物中叶下珠素、隐叶下珠素、尼替拉宁和尼拉宁的分布。

方法

采用 XBridge 柱（150×4.6mm，5.0μm id）在等度洗脱模式下，以乙腈和水的混合物为流动相，含 TFA（0.05%，v/v，pH=2.15），在环境温度下以 1mL/min 的流速进行分离。

结果

叶下珠素、隐叶下珠素、尼替拉宁和尼拉宁分别在 10.47、11.10、13.67 和 14.53min 的平均保留时间洗脱。所有四种分析物的检出限和定量限分别为 0.75 和 3.00μg/mL。叶下珠素、隐叶下珠素、尼替拉宁和尼拉宁的日内和日间精密度的 RSDr 值分别为 0.38-1.32%和 0.45-1.77%；0.22-3.69%和 0.24-3.04%、0.73-2.37%和 0.09-0.31%、1.56-2.77%和 0.12-0.68%。