Defence Institute of Physiology and Allied Sciences (DIPAS), Defence R&D Organization (DRDO), Lucknow Road, Timarpur, Delhi 110054, India.
Database (Oxford). 2020 Dec 1;2020. doi: 10.1093/database/baaa101.
Around 140 million people live in high-altitude (HA) conditions! and even a larger number visit such places for tourism, adventure-seeking or sports training. Rapid ascent to HA can cause severe damage to the body organs and may lead to many fatal disorders. During induction to HA, human body undergoes various physiological, biochemical, hematological and molecular changes to adapt to the extreme environmental conditions. Several literature references hint that gene-expression-regulation and regulatory molecules like miRNAs and transcription factors (TFs) control adaptive responses during HA stress. These biomolecules are known to interact in a complex combinatorial manner to fine-tune the gene expression and help in controlling the molecular responses during this stress and ultimately help in acclimatization. High-Altitude Human miRNA Database (HAHmiR.DB) is a unique, comprehensive and curated collection of miRNAs that have been experimentally validated to be associated with HA stress, their level of expression in different altitudes, fold change, experiment duration, biomarker association, disease and drug association, tissue-specific expression level, Gene Ontology (GO) and Kyoto Encyclopaedia of Gene and Genomes (KEGG) pathway associations. As a server platform, it also uniquely constructs and analyses interactive miRNA-TF-gene coregulatory networks and extracts regulatory circuits/feed-forward loops (FFLs). These regulatory circuits help to offer mechanistic insights into complex regulatory mechanisms during HA stress. The server can also build these regulatory networks between two and more miRNAs of the database and also identify the regulatory circuits from this network. Hence, HAHmiR.DB is the first-of-its-kind database in HA research, which is a reliable platform to explore, compare, analyse and retrieve miRNAs associated with HA stress, their coregulatory networks and FFL regulatory-circuits. HAHmiR.DB is freely accessible at http://www.hahmirdb.in.
约有 1.4 亿人生活在高海拔（HA）地区！还有更多的人出于旅游、探险或运动训练的目的而前往这些地方。快速上升到高海拔地区可能会对身体器官造成严重损害，并可能导致许多致命疾病。在向高海拔地区诱导适应的过程中，人体会经历各种生理、生化、血液和分子变化，以适应极端的环境条件。有几篇文献指出，基因表达调控和调节分子，如 miRNA 和转录因子（TFs），控制着高海拔应激时的适应性反应。这些生物分子以复杂的组合方式相互作用，以精细调节基因表达，并有助于控制该应激期间的分子反应，最终有助于适应。高海拔人类 miRNA 数据库（HAHmiR.DB）是一个独特的、全面的、经过精心整理的 miRNA 集合，这些 miRNA 已被实验验证与高海拔应激相关，它们在不同海拔高度的表达水平、倍数变化、实验持续时间、生物标志物关联、疾病和药物关联、组织特异性表达水平、基因本体论（GO）和京都基因与基因组百科全书（KEGG）途径关联。作为一个服务器平台，它还独特地构建和分析了相互作用的 miRNA-TF-基因核心调控网络，并提取了调控回路/前馈环（FFL）。这些调控回路有助于提供高海拔应激期间复杂调控机制的机制见解。该服务器还可以在数据库的两个或更多 miRNA 之间构建这些调控网络，并从该网络中识别出调控回路。因此，HAHmiR.DB 是高海拔研究领域的首个此类数据库，它是一个可靠的平台，用于探索、比较、分析和检索与高海拔应激相关的 miRNA、它们的核心调控网络和 FFL 调控回路。HAHmiR.DB 可免费访问：http://www.hahmirdb.in。
