Naso Luciana G, Martínez Valeria R, Ferrer Evelina G, Williams Patricia A M
CEQUINOR-CONICET-CICPBA-UNLP, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Bv. 120 N° 1465, 1900 La Plata, Argentina.
CEQUINOR-CONICET-CICPBA-UNLP, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Bv. 120 N° 1465, 1900 La Plata, Argentina.
J Trace Elem Med Biol. 2021 Mar;64:126690. doi: 10.1016/j.jtemb.2020.126690. Epub 2020 Nov 20.
Non-small-cell lung cancer (NSCLC) is the most frequent type of lung cancer and more than 90 % of mortality is due to metastasis-related deaths. Flavonoids are considered nutraceuticals due to the variety of pharmacological properties. In this paper, we studied the effects of baicalin, silibinin, apigenin, luteolin, and its oxidovanadium(IV) cation complexes on the viability, adhesion to fibronectin, invasion, and migration on human lung cancer cell line A549. In addition, in order to complete the study of the interaction of VOflavonoids and bovine serum albumin (BSA), the binding ability of silibinin and VOsil to the protein was evaluated.
To establish the non-cytotoxic concentration range of the tested compounds, the cancer cell viability was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Cell migration and invasion assays were performed using Boyden chambers and adhesion assay using MTT method. The interaction of compounds with BSA were investigated in physiological buffer (pH = 7.4) by fluorescence spectroscopy.
All complexes inhibited the metastatic cascade steps to a greater extent than their respective ligands. Likewise, based on binding constant values (K) for BSA-silibinin and BSA-VOsil, we can suggest that both compounds can interact with the protein.
Although all the complexes suppressed cell adhesion, invasion and migration, VOlut can be considered as a good candidate to continue the trials because it presented encouraging results as a potential antitumor and antimetastatic agent, and can be transported by BSA.
非小细胞肺癌（NSCLC）是最常见的肺癌类型，超过90%的死亡是由转移相关的死亡导致的。由于具有多种药理特性，黄酮类化合物被视为营养保健品。在本文中，我们研究了黄芩苷、水飞蓟宾、芹菜素、木犀草素及其氧化钒（IV）阳离子络合物对人肺癌细胞系A549的活力、与纤连蛋白的粘附、侵袭和迁移的影响。此外，为了完成对VO黄酮类化合物与牛血清白蛋白（BSA）相互作用的研究，评估了水飞蓟宾和VOsil与该蛋白质的结合能力。
为了确定受试化合物的无细胞毒性浓度范围，使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四氮唑（MTT）法评估癌细胞活力。使用博伊登小室进行细胞迁移和侵袭试验，并使用MTT法进行粘附试验。通过荧光光谱法在生理缓冲液（pH = 7.4）中研究化合物与BSA的相互作用。
所有络合物对转移级联步骤的抑制程度均大于其各自的配体。同样，基于BSA-水飞蓟宾和BSA-VOsil的结合常数（K）值，我们可以认为这两种化合物都能与该蛋白质相互作用。
尽管所有络合物都抑制了细胞粘附、侵袭和迁移，但VOlut可被视为继续试验的良好候选物，因为它作为一种潜在的抗肿瘤和抗转移剂呈现出令人鼓舞的结果，并且可以由BSA运输。
