Department of Biomedical Engineering, Research Center for Nano-biomaterials and Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, People's Republic of China.
Shanxi Key Laboratory of Material Strength and Structural Impact, Institute of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, People's Republic of China.
Biomed Mater. 2021 Mar 1;16(3). doi: 10.1088/1748-605X/abcf5c.
Circulating tumor cells (CTCs) are regarded as an effective biomarker for cancer detection, diagnosis and prognosis monitoring. CTCs capture based on nanostructured substrates is a powerful technique. Some specific adhesion molecule antibody coated on the surface of nanostructured substrates, such as EpCAM, is commonly used to enhance the CTCs capture efficiency. Substrate nanotopographies regulate the interaction between the substrates and captured cells, further influencing cell capture efficiency. However, the relationship between cell capture efficiency and cell-substrate interaction remains poorly understood. Here, we explored the relationship between cell capture efficiency and cell-substrate interaction based on two sets of nanostructures with different nanotopographies without antibody conjugation. Given the urgent demand for improving the capture efficiency of EpCAM-negative cells, we used HeLa (EpCAM-negative) cells as the main targets. We demonstrated that HeLa cells could be more effectively captured by two nanostructural substrates, especially by double-layer composite nanoforests. Therefore, the morphological and migrating interaction between HeLa cells and distinct substrates was associated with cell capture efficiency. Our findings demonstrated the potential mechanism for optimizing the nanotopography for higher capture efficiency, and provide a potential foundation for cancer detection, diagnosis and treatment.
循环肿瘤细胞 (CTCs) 被认为是癌症检测、诊断和预后监测的有效生物标志物。基于纳米结构基底的 CTC 捕获是一种强大的技术。一些特定的粘附分子抗体涂覆在纳米结构基底的表面上，例如 EpCAM，通常用于提高 CTC 的捕获效率。基底的纳米形貌调节了基底与捕获细胞之间的相互作用，进一步影响细胞的捕获效率。然而，细胞捕获效率与细胞-基底相互作用之间的关系仍不清楚。在这里，我们探索了两组具有不同纳米形貌且没有抗体结合的纳米结构的基础上，细胞捕获效率与细胞-基底相互作用之间的关系。鉴于提高 EpCAM 阴性细胞捕获效率的迫切需求，我们使用 HeLa（EpCAM 阴性）细胞作为主要研究对象。我们证明了 HeLa 细胞可以通过两种纳米结构基底更有效地被捕获，特别是双层复合纳米森林。因此，HeLa 细胞与不同基底之间的形态和迁移相互作用与细胞捕获效率相关。我们的研究结果揭示了优化纳米形貌以提高捕获效率的潜在机制，并为癌症检测、诊断和治疗提供了潜在的基础。
