野猪体内多房棘球绦虫的分子诊断病例研究——兼论其在土耳其的公共卫生意义。

A Case-Study of the Molecular Diagnosis of Echinococcus multilocularis in Wild Boar with Comments on its Public Health Significance in Turkey.

机构信息

Department of Parasitology, Faculty of Veterinary Medicine, Bingol University, Bingol, 12000, Turkey.

Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119, Elazig, Turkey.

出版信息

J Parasitol. 2020 Nov 12;106(6):730-734. doi: 10.1645/19-196.

DOI:10.1645/19-196

PMID:33260210

Abstract

Echinococcus multilocularis is a parasite species of zoonotic importance which can be fatal to humans and causes Alveolar Echinococcosis (AE). This report describes the development of a cyst from the liver of a wild boar and molecular confirmation of its identification. The cyst material was obtained from the liver of a wild boar killed by hunters. Genomic DNA was extracted from the germinal layer of the cyst material, and 875 bp mitochondrial cytochrome c oxidase subunit I (COI) gene fragment was amplified by PCR and sequenced. A BLAST search matched 100% with published Echinococcus multilocularis sequences. This study confirms the occurrence of E. multilocularis in a wild boar for the first time in Turkey.

摘要

泡状棘球蚴是一种具有动物源性重要性的寄生虫物种，对人类有致命性，并导致泡型包虫病（AE）。本报告描述了从野猪肝脏中发育的囊肿，并对其鉴定进行了分子确认。囊肿材料取自被猎人杀死的野猪的肝脏。从囊肿材料的生殖层中提取基因组 DNA，通过 PCR 扩增并测序 875bp 线粒体细胞色素 c 氧化酶亚基 I（COI）基因片段。BLAST 搜索与已发表的多房棘球绦虫序列完全匹配。本研究首次在土耳其证实野猪中存在多房棘球绦虫。