Calvet M C, Calvet J
INSERM U 249, CNRS LP8402, EPHE, Institut de Biologie, Montpellier, France.
Rev Electroencephalogr Neurophysiol Clin. 1987 Dec;17(4):437-46. doi: 10.1016/s0370-4475(87)80089-8.
The electrophysiological and morphological properties of the Purkinje cells (P cells) grown in organotypic cultures were studied in HRP intracellulary labelled neurons and compared to Golgi stained P cells from kitten of different ages. The effects of afferent fiber depletions on the final dendritic topological parameters were investigated by 3-dimensional reconstructions and computerized methods. In culture, the P cell dendritic trees always appeared reduced in size when compared to their in vivo counterparts. A discriminant analysis permitted the recognition of 3 populations of cultured P cells according to the type of deafferentation. In intact animals, the dendritic organization appeared biplanar around day 13. These results demonstrate that the presence of all normal inputs is required to achieve the full elaboration and the monoplanar disposition of the P cell dendrites.
在器官型培养中生长的浦肯野细胞（P细胞）的电生理和形态学特性，在辣根过氧化物酶（HRP）细胞内标记的神经元中进行了研究，并与来自不同年龄小猫的高尔基染色P细胞进行了比较。通过三维重建和计算机化方法研究了传入纤维缺失对最终树突拓扑参数的影响。在培养中，与体内对应物相比，P细胞树突总是显得尺寸减小。判别分析允许根据去传入的类型识别3种培养的P细胞群体。在完整动物中，树突组织在第13天左右呈现双平面。这些结果表明，需要所有正常输入的存在才能实现P细胞树突的充分发育和单平面排列。
