Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Research Group on Sensory Evaluation and Consumer Preference of Horticultural Products, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Molecules. 2020 Nov 27;25(23):5567. doi: 10.3390/molecules25235567.
Metabolites play vital roles in shaping the quality of fresh fruit. In this study, Korla pear fruit harvested from twelve orchards in South Xinjiang, China, were ranked in sensory quality by fuzzy logic sensory evaluation for two consecutive seasons. Then, gas chromatography-mass spectrometry (GC-MS) was applied to determine the primary metabolites and volatile compounds. Sensory evaluation results showed that the panelists were more concerned about 'mouth feel' and 'aroma' than about 'fruit size', 'fruit shape' and 'peel color'. In total, 20 primary metabolites and 100 volatiles were detected in the pear fruit. Hexanal, ()-2-hexenal, nonanal, d-limonene, ()-3-hexen-1-yl acetate and hexyl acetate were identified as the major volatile compounds. Correlation analysis revealed that l-(+)-tartaric acid, hexanoic acid, trans-limonene oxide and 2,2,4-trimethyl-1,3-pentanediol diisobutyrate were negatively correlated with sensory scores. Furthermore, OPLS-DA results indicated that the fruit from three orchards with lower ranks in quality could be distinguished from other samples based on the contents of l-(+)-tartaric acid and other eight metabolites, which were all associated with 'mouth feel' and 'aroma'. This study reveals the metabolites that might be closely associated with the sensory quality attributes of Korla pear, which may provide some clues for promoting the fruit quality in actual production.
代谢物在塑造新鲜水果品质方面发挥着重要作用。本研究连续两个季节采用模糊逻辑感官评价对来自中国新疆南部 12 个果园的库尔勒梨果实进行感官质量排序。然后,应用气相色谱-质谱联用(GC-MS)法测定了主要代谢物和挥发性化合物。感官评价结果表明,品评员更关注“口感”和“香气”,而不是“果实大小”、“果实形状”和“果皮颜色”。总共检测到梨果实中的 20 种主要代谢物和 100 种挥发性化合物。鉴定出的主要挥发性化合物有己醛、()-2-己烯醛、壬醛、柠檬烯、()-3-己烯-1-基乙酸酯和己酸乙酯。相关性分析表明,L-(+)-酒石酸、己酸、反式-柠檬烯氧化物和 2,2,4-三甲基-1,3-戊二醇二异丁酸酯与感官评分呈负相关。此外,OPLS-DA 结果表明,三个感官质量等级较低的果园的果实可以根据 L-(+)-酒石酸和其他八种与“口感”和“香气”相关的代谢物的含量与其他样品区分开来。本研究揭示了可能与库尔勒梨感官品质属性密切相关的代谢物,这可能为实际生产中促进果实品质提供一些线索。