A. Öman, MD, L. Berntson, MD, Associate Professor, Department of Women's and Children's Health, Uppsala University, Uppsala;
J. Dicksved, PhD, Associate Professor, Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Uppsala.
J Rheumatol. 2021 Oct;48(10):1589-1595. doi: 10.3899/jrheum.200551. Epub 2020 Dec 1.
Changes in the composition of gut microbiota have been suggested to be associated with juvenile idiopathic arthritis (JIA). The objective in this study was to investigate if the diversity and composition of the fecal microbiota differed between children with JIA and healthy controls (HCs), and if the microbiota differed between children with JIA and their healthy siblings.
In this multicenter, case-control study, fecal samples were collected from 75 children with JIA and 32 HCs. Eight of the HCs were siblings to 8 children with JIA, and they were compared only pairwise with their siblings. The microbiota was determined using sequencing amplicons from the V3 and V4 regions of the 16S rRNA gene. Alpha diversity, community composition of microbiota, and relative abundances of taxa were compared between children with JIA and healthy unrelated controls as well as between children with JIA and healthy siblings.
Our data revealed no significant differences in α-diversity or community composition of microbiota between children with JIA, healthy unrelated controls, or healthy siblings. Analyses of relative abundances of phyla, families, and genera identified trends of differing abundances of some taxa in children with JIA, in comparison with both HCs and healthy siblings, but none of these findings were significant after adjustment for multiple comparisons.
There were no significant differences in the composition of fecal microbiota in children with JIA compared with HCs. The composition of microbiota in children with JIA did not differ significantly from that in their healthy siblings.
肠道微生物群的组成变化被认为与幼年特发性关节炎（JIA）有关。本研究旨在探讨 JIA 患儿与健康对照（HCs）之间粪便微生物群的多样性和组成是否存在差异，以及 JIA 患儿与其健康兄弟姐妹之间的微生物群是否存在差异。
在这项多中心病例对照研究中，采集了 75 名 JIA 患儿和 32 名 HCs 的粪便样本。其中 8 名 HCs 是 8 名 JIA 患儿的兄弟姐妹，仅对他们与兄弟姐妹进行了两两比较。使用 16S rRNA 基因 V3 和 V4 区域的扩增子进行微生物组测定。比较 JIA 患儿与健康无关对照组以及 JIA 患儿与健康兄弟姐妹之间的α多样性、微生物群组成以及分类群的相对丰度。
我们的数据显示，JIA 患儿、健康无关对照组或健康兄弟姐妹之间的α多样性或微生物群组成没有显著差异。对门、科和属的相对丰度进行分析，发现与 HCs 和健康兄弟姐妹相比，JIA 患儿中有一些分类群的丰度存在差异的趋势，但在进行多次比较调整后，这些发现均无统计学意义。
与 HCs 相比，JIA 患儿粪便微生物群的组成没有显著差异。JIA 患儿的微生物群组成与他们的健康兄弟姐妹没有显著差异。
