Long Non-Coding RNA SNHG7 Alleviates Oxygen and Glucose Deprivation/Reoxygenation-Induced Neuronal Injury by Modulating miR-9/SIRT1 Axis in PC12 Cells: Potential Role in Ischemic Stroke.

OBJECTIVE

The roles of long non-coding RNA (lncRNAs) in ischemic stroke (IS) have been widely illustrated. Here, we focused on the function and mechanism of lncRNA in IS.

METHODS

Middle cerebral artery occlusion (MCAO) was used for inducing to establish IS models in vivo. Oxygen and glucose deprivation/reoxygenation (OGD/R) was used for treating PC12 cells to establish IS models in vitro. Relative expression of and was determined by qRT-PCR. The neuronal injury was assessed by measuring relative activity of ROS, malondialdehyde (MDA) level and cell viability. Cell viability was determined by MTT assay. Dual-luciferase reporter (DLR) assay was employed to test the target of or . Western blot was used to determine the protein expression of . Apoptosis rate was measured by flow cytometry.

RESULTS

was down-regulated and 9 was up-regulated by MCAO treatment in brain tissues of and by OGD/R treatment in PC12 cells. Overexpression of or suppression of decreased the relative activity of ROS and the MDA level as well as enhancing cell viability, and reduced apoptosis rate in OGD/R-induced PC12 cells (IS cells). was targeted by and was targeted by . The protein expression of was reduced by OGD/R treatment in PC12 cells. The suppressive effects of on the relative activity of ROS, the MDA level and apoptosis rate as well as the promotion effect of on cell viability were reversed by mimics or sh- in IS cells.

CONCLUSION

LncRNA alleviated OGD/R-induced neuronal injury by mediating / axis in vitro.