School of Life Sciences, BK21 Four KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, Republic of Korea.
Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 55365, Republic of Korea.
J Microbiol Biotechnol. 2021 Feb 28;31(2):189-196. doi: 10.4014/jmb.2010.10058.
Periplanetasin-2 from cockroach exhibits broad-spectrum antimicrobial activity. The underlying antibacterial mechanisms rely on the stimulation of reactive oxygen species overproduction to induce apoptotic cell death. A promising strategy to increase the bioavailability of periplanetasin-2 involves reducing the dose through combination therapy with other antibacterials that show synergistic effects. Thus, the synergistic antibacterial activity of periplanetasin-2 with conventional antibacterial agents and its mechanisms was examined against in this study. Among the agents tested, the combinations of periplanetasin-2 with vancomycin and chloramphenicol exhibited synergistic effects. Periplanetasin-2 in combination with vancomycin and chloramphenicol demonstrated antibacterial activity through the intracellular oxidative stress response. The combination with vancomycin resulted in the enhancement of bacterial apoptosislike death, whereas the combination with chloramphenicol enhanced oxidative stress damage. These synergistic interactions of periplanetasin-2 can help broaden the spectrum of conventional antibiotics. The combination of antimicrobial peptides and conventional antibiotics is proposed as a novel perspective on treatments to combat severe bacterial infection.
蜚蠊素-2 具有广谱的抗菌活性。其潜在的抗菌机制依赖于活性氧过度产生的刺激，诱导细胞凋亡死亡。增加蜚蠊素-2 生物利用度的一种有前途的策略是通过与表现出协同作用的其他抗菌药物联合治疗来减少剂量。因此，本研究检测了蜚蠊素-2 与传统抗菌药物的协同抗菌活性及其机制。在测试的药物中，蜚蠊素-2 与万古霉素和氯霉素的组合表现出协同作用。通过细胞内氧化应激反应，蜚蠊素-2 与万古霉素和氯霉素联合具有抗菌活性。与万古霉素联合使用会增强细菌类似凋亡的死亡，而与氯霉素联合使用会增强氧化应激损伤。蜚蠊素-2 的这种协同相互作用可以帮助扩大传统抗生素的谱。建议将抗菌肽与传统抗生素联合使用作为治疗严重细菌感染的新视角。
