Unidad de Investigaciones en Salud Pública, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, Mexico.
Departamento de Investigación, Hospital de la Mujer de Culiacán, Servicios de Salud de Sinaloa, Culiacán, Sinaloa, Mexico.
Cytokine. 2021 Feb;138:155362. doi: 10.1016/j.cyto.2020.155362. Epub 2020 Nov 29.
The pregnancy period represents the most intense period of growth and development. Pre-pregnancy weight influences weight gain during pregnancy. Leptin is a hormone mainly derived from white adipose tissue, during pregnancy leptin is also produced by the placenta. It has been suggested that the effects of placental leptin on the mother may contribute to endocrine-mediated alterations in energy balance; a dysregulation in leptin levels or its receptors may lead to poor birth outcomes. Therefore, the main goal of the present study was to analyze the differences in birth outcomes by maternal weight with the expression level of leptin receptor in maternal peripheral blood mononuclear cell (PBMC) and placental tissue.
Women with full-term gestation and its offspring were enrolled. Total RNA from maternal PBMC and placenta was obtained to perform the analysis of expression of the leptin receptor (LEPR) gene trough real-time PCR technique. Data were analyzed using one-way ANOVA or Mann-Whitney u test when applicable. Pearson correlation coefficient was used to determine the relationship between continuous variables (Stata v.13); p ≤ 0.05 was considered statistically significant.
No statistically significant differences were found between LEPR expression level and the BMI studied groups in maternal PBMC and placental tissue. Interaction between gestational weight gain (GWG) and LEPR in maternal PBMC explain in a 32% the variability of the newborn weight.
LEPR expression level in maternal PBMC correlates with newborn measurements independent from sex. GWG can affect fetal development by increasing fetal birth weight.
妊娠期间是生长和发育最旺盛的时期。孕前体重会影响孕期体重的增加。瘦素是一种主要来源于白色脂肪组织的激素，在怀孕期间，胎盘也会产生瘦素。有人认为，胎盘瘦素对母亲的影响可能有助于内分泌介导的能量平衡改变；瘦素水平或其受体的失调可能导致不良的出生结局。因此，本研究的主要目的是分析母体外周血单个核细胞（PBMC）和胎盘组织中瘦素受体（LEPR）表达水平与母体体重差异对出生结局的影响。
纳入足月妊娠及其后代的女性。从母体 PBMC 和胎盘获得总 RNA，通过实时 PCR 技术分析瘦素受体（LEPR）基因的表达。当适用时，使用单向方差分析或曼-惠特尼 U 检验分析数据。使用 Pearson 相关系数确定连续变量之间的关系（Stata v.13）；p ≤ 0.05 被认为具有统计学意义。
在母体 PBMC 和胎盘组织中，LEPR 表达水平与研究的 BMI 组之间未发现统计学差异。母体 PBMC 中 GWG 和 LEPR 之间的相互作用解释了新生儿体重变异性的 32%。
母体 PBMC 中的 LEPR 表达水平与新生儿的测量值独立于性别相关。GWG 通过增加胎儿出生体重而影响胎儿发育。
