Gu Ruiting, Lin Haiying, Zhou Yi, Song Xiaoyue, Xu Shaochun, Yue Shidong, Zhang Yu, Xu Shuai, Zhang Xiaomei
CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China.
J Hazard Mater. 2021 Feb 5;403:123875. doi: 10.1016/j.jhazmat.2020.123875. Epub 2020 Sep 8.
Seagrass meadows are recognized as crucial and are among the most vulnerable habitats worldwide. The aquatic plant genus Ruppia is tolerant of a wide salinity range, and high concentrations of trace metals. However, the tolerance of its early life stages to such trace metal exposure is unclear. Thus, the current study investigated the trace metal-absorbing capacity of three different life-history stages of Ruppia sinensis, a species that is widely distributed in China, by observing toxic symptoms at the individual, subcellular, and transcription levels. The seedling period was the most vulnerable, with visible toxic effects at the individual level in response to 50 μM copper and 500 μM cadmium after 4 days of exposure. The highest concentrations of trace metals occurred in the vacuoles and cytoplasmic structures of aboveground tissues. Genes related to signal identification and protein processing were significantly downregulated after 4 days of exposure to copper and cadmium. These results provide information relating to the strategies evolved by R. sinensis to absorb and isolate trace elements, and highlight the phytoremediation potential of this species.
海草草甸被认为至关重要，且是全球最脆弱的栖息地之一。水生植物川蔓藻属能耐受较宽的盐度范围和高浓度的痕量金属。然而，其早期生命阶段对这种痕量金属暴露的耐受性尚不清楚。因此，本研究通过在个体、亚细胞和转录水平观察毒性症状，研究了在中国广泛分布的中华川蔓藻三个不同生活史阶段的痕量金属吸收能力。幼苗期最为脆弱，暴露4天后，在个体水平上，对50 μM铜和500 μM镉出现了明显的毒性效应。痕量金属的最高浓度出现在地上组织的液泡和细胞质结构中。暴露于铜和镉4天后，与信号识别和蛋白质加工相关的基因显著下调。这些结果提供了有关中华川蔓藻吸收和隔离微量元素所采用策略的信息，并突出了该物种的植物修复潜力。
