Heath-Fracica L A, Estévez E G
Clinical Microbiology Laboratory, Duke University Medical Center, Durham, NC 27710.
Diagn Microbiol Infect Dis. 1987 Sep;8(1):25-30. doi: 10.1016/0732-8893(87)90043-5.
A liposome-enhanced latex agglutination test (Leapstrep, Cooper Biomedical, Inc., Malvern, PA) was evaluated for its efficacy as a screening test for antistreptolysin O (ASO) and antideoxyribonuclease B (ADNB) antibodies. A total of 179 sera were tested by both Leapstrep and reference ASO and ADNB test methods. The Leapstrep test had a sensitivity of 77% and a specificity of 69% for ASO antibodies when compared to a microtiter adaptation of the Todd-Hewitt ASO tube test. It had a sensitivity of 76% and 75% specificity for ADNB antibodies as compared to the ADNB microtiter enzyme neutralization test. The overall sensitivity of Leapstrep for detecting ASO and/or ADNB antibodies was 73%. The Leapstrep test failed to detect elevated levels of ASO and/or ADNB antibodies in eight patients with streptococcal infections or poststreptococcal sequelae. The Leapstrep test appears to have insufficient sensitivity to be recommended as a screening test for streptococcal antibodies.
对一种脂质体增强乳胶凝集试验（Leapstrep，库珀生物医学公司，宾夕法尼亚州马尔文）作为抗链球菌溶血素O（ASO）和抗脱氧核糖核酸酶B（ADNB）抗体筛查试验的效果进行了评估。采用Leapstrep以及参考ASO和ADNB检测方法对总共179份血清进行了检测。与托德 - 休伊特ASO试管试验的微量滴定法相比，Leapstrep试验对ASO抗体的敏感性为77%，特异性为69%。与ADNB微量滴定酶中和试验相比，它对ADNB抗体的敏感性为76%，特异性为75%。Leapstrep检测ASO和/或ADNB抗体的总体敏感性为73%。Leapstrep试验未能检测出8例链球菌感染或链球菌感染后后遗症患者中升高的ASO和/或ADNB抗体水平。Leapstrep试验的敏感性似乎不足，不推荐作为链球菌抗体的筛查试验。
