Key Laboratory of Neuroregeneration of Jiangsu and the Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China.
Department of Physiology, School of medicine, Nantong University, Nantong, China.
J Neurosci Res. 2021 Mar;99(3):843-857. doi: 10.1002/jnr.24763. Epub 2020 Dec 3.
RNA methylation is involved in multiple physiological and pathological processes. However, the role of RNA methylation in spinal cord regeneration has not been reported. In this study, we find an altered m6A (N6-methyladenosine) RNA methylation profiling following zebrafish spinal cord injury (SCI), in line with an altered transcription level of the m6A methylase Mettl3. Interestingly, many of the differential m6A-tagged genes associated with neural regeneration are hypomethylated, but their transcription levels are upregulated in SCI. Moreover, we find that METTL3 may be important for spinal cord regeneration. We also show a conserved feature of METTL3 changes in mouse SCI model, in which the expression of METTL3 is increased in both astrocytes and neural stem cells. Together, our results indicate that m6A RNA methylation is dynamic and conserved following SCI and may contribute to spinal cord regeneration.
RNA 甲基化参与多种生理和病理过程。然而，RNA 甲基化在脊髓再生中的作用尚未被报道。在这项研究中，我们发现斑马鱼脊髓损伤后 m6A（N6-甲基腺苷）RNA 甲基化谱发生改变，与 m6A 甲基转移酶 Mettl3 的转录水平改变一致。有趣的是，许多与神经再生相关的差异 m6A 标记基因的甲基化程度降低，但在 SCI 中其转录水平上调。此外，我们发现 METTL3 可能对脊髓再生很重要。我们还在小鼠 SCI 模型中观察到 METTL3 变化的保守特征，其中星形胶质细胞和神经干细胞中的 METTL3 表达增加。总之，我们的结果表明，m6A RNA 甲基化在 SCI 后是动态和保守的，可能有助于脊髓再生。
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