State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
Biochem Biophys Res Commun. 2021 Jan 1;534:206-211. doi: 10.1016/j.bbrc.2020.11.111. Epub 2020 Dec 1.
Nucleotide-binding site (NBS) and leucine-rich repeat (LRR) receptors (NLRs) play important roles in plant immunity. The genome of Arabidopsis thaliana contains about 150 genes encoding NLR proteins, but few of them have been studied. We transiently expressed a series of NBS-LRR proteins in the leaves of Nicotiana benthamiana, and found that the CC-NBS-LRR protein (AT1G12290) was able to trigger cell death, a characterized function for the activation of an NLR protein. We observed that the YFP-tagged AT1G12290 was localized on the plasma membrane (PM), and the predicted myristoylation site Gly2 is required for the localization and function of the protein. Further structure dissection revealed that the CC domain was enough to activate cell death, and the N-terminal 1-100 amino acid fragment was the minimal region to induce cell death and self-association. Our research provides important clues to elucidate the activation mechanism of AT1G12290.
核苷酸结合位点（NBS）和富含亮氨酸重复（LRR）受体（NLRs）在植物免疫中发挥重要作用。拟南芥基因组包含约 150 个编码 NLR 蛋白的基因，但其中很少有被研究过。我们在本氏烟叶片中瞬时表达了一系列 NBS-LRR 蛋白，发现 CC-NBS-LRR 蛋白（AT1G12290）能够触发细胞死亡，这是 NLR 蛋白激活的一个特征功能。我们观察到，YFP 标记的 AT1G12290 定位于质膜（PM）上，并且预测的豆蔻酰化位点 Gly2 是该蛋白定位和功能所必需的。进一步的结构分析表明，CC 结构域足以激活细胞死亡，而 N 端 1-100 个氨基酸片段是诱导细胞死亡和自组装的最小区域。我们的研究为阐明 AT1G12290 的激活机制提供了重要线索。
