Erdogan Burcu, Bearce Elizabeth A, Lowery Laura Anne
Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Institute of Molecular Biology, Department of Biology, University of Oregon, Eugene, Oregon 97403, USA.
Cold Spring Harb Protoc. 2021 Apr 1;2021(4):pdb.prot104463. doi: 10.1101/pdb.prot104463.
The cytoskeleton is a dynamic, fundamental network that not only provides mechanical strength to maintain a cell's shape but also controls critical events like cell division, polarity, and movement. Thus, how the cytoskeleton is organized and dynamically regulated is critical to our understanding of countless processes. Live imaging of fluorophore-tagged cytoskeletal proteins allows us to monitor the dynamic nature of cytoskeleton components in embryonic cells. Here, we describe a protocol to monitor and analyze cytoskeletal dynamics in primary embryonic neuronal growth cones and neural crest cells obtained from embryos.
细胞骨架是一个动态的基本网络，它不仅提供机械强度以维持细胞形状，还控制诸如细胞分裂、极性和运动等关键事件。因此，细胞骨架如何组织和动态调节对于我们理解无数过程至关重要。对荧光团标记的细胞骨架蛋白进行实时成像，使我们能够监测胚胎细胞中细胞骨架成分的动态特性。在这里，我们描述了一种用于监测和分析从胚胎获得的原代胚胎神经元生长锥和神经嵴细胞中细胞骨架动力学的方案。
