Department of Food and Nutrition, Gyeongsang National University, Jinju, Republic of Korea.
Research Group of Consumer Safety, Korea Food Research Institute, Wanju-gun, Jeollabuk-do, Republic of Korea.
Sci Rep. 2020 Dec 3;10(1):21090. doi: 10.1038/s41598-020-77868-4.
Bacteria can form biofilms, complex microbial communities protected from environmental stress, on food contact surfaces. Brassicaceae plant has been shown to contain bioactive compounds with antimicrobial activities. The objective of this study was to evaluate the synergistic effects of Brassicaceae species and proteinase K against E. coli O157:H7 biofilm. We determined the minimum biofilm inhibitory concentration, the fractional inhibitory concentration indexes, and the synergistic inhibitory effect of Raphanus sativus var. longipinnatus, R. sativus, and Brassica oleracea var. acephala extracts with proteinase K on E. coli O157:H7. The biofilm showed a 49% reduction with 2 mg/mL R. sativus. The combination of proteinase K 25 µg/mL significantly increased the effect of 2 mg/mL R. sativus var. longipinnatus and the combined treatment yielded up to 2.68 log reduction on stainless steel coupons. The results showed that the combination of R. sativus var. longipinnatus extract and proteinase K could serve as an anti-biofilm agent with synergistic effects for inhibiting E. coli O157:H7 biofilm on stainless steel surfaces.
细菌可以在食品接触表面形成生物膜，这是一种受环境压力保护的复杂微生物群落。已经证明芸苔属植物含有具有抗菌活性的生物活性化合物。本研究的目的是评估芸苔属植物物种和蛋白酶 K 对大肠杆菌 O157:H7 生物膜的协同作用。我们确定了最小生物膜抑制浓度、部分抑制浓度指数以及蛋白酶 K 对大肠杆菌 O157:H7 的协同抑制作用Raphanus sativus var. longipinnatus、R. sativus 和 Brassica oleracea var. acephala 提取物的生物膜抑制作用。2 mg/mL 的萝卜表现出 49%的减少。25 µg/mL 的蛋白酶 K 组合显著增加了 2 mg/mL 的萝卜 var. longipinnatus 的作用，联合处理在不锈钢试片上产生了高达 2.68 的对数减少。结果表明，萝卜 var. longipinnatus 提取物和蛋白酶 K 的组合可以作为一种具有协同作用的抗生物膜剂，抑制不锈钢表面上的大肠杆菌 O157:H7 生物膜。
