Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Asian Pac J Allergy Immunol. 2023 Dec;41(4):401-406. doi: 10.12932/AP-140720-0913.
Cytokine-induced killer (CIK) cells are ex-vivo expanded T cells which present a phenotype of both T and Natural Killer cell properties.
To compare the proliferation and functional properties of human CIK cells cultured in three cell culture plasticwares.
The number and viability of CIK cells were monitored. The expression of surface markers (CD3 and CD56), TH1 cytokines (IFN-γ and TNF-α), and cytolytic granules (granzyme B and perforin) were determined by flow cytometry.
The number of CIK cells cultured in a static bag was highest compared to those in a petri dish and gas-permeable flask. However, CIK cells cultured in all plasticwares similarity expressed surface marker, TH1 cytokines, and cytolytic granules.
Considering safety, efficacy, and cost, a static bag is the best plasticware for culturing CIK cells.
细胞因子诱导的杀伤（CIK）细胞是体外扩增的 T 细胞，具有 T 细胞和自然杀伤细胞特性的表型。
比较三种细胞培养塑料器皿中培养的人 CIK 细胞的增殖和功能特性。
监测 CIK 细胞的数量和活力。通过流式细胞术测定表面标志物（CD3 和 CD56）、TH1 细胞因子（IFN-γ 和 TNF-α）和细胞毒性颗粒（颗粒酶 B 和穿孔素）的表达。
与培养在培养皿和透气瓶中的细胞相比，静态袋中培养的 CIK 细胞数量最高。然而，在所有塑料器皿中培养的 CIK 细胞相似地表达了表面标志物、TH1 细胞因子和细胞毒性颗粒。
考虑到安全性、疗效和成本，静态袋是培养 CIK 细胞的最佳塑料器皿。
