The RAD50 gene of Saccharomyces cerevisiae is not essential for vegetative growth.
作者信息
Kupiec M
机构信息
Department of Genetics, Hebrew University, Jerusalem, Israel.
出版信息
Curr Genet. 1986;10(6):487-9. doi: 10.1007/BF00419878.
PMID:3327607
Abstract
The gene RAD50 was located by the ability of subclones to restore the Rad+ phenotype following transformation of a rad50-1 mutant. Disruption of the gene was achieved by directed integration of a plasmid carrying a fragment internal to RAD50. Haploids with the disrupted gene are viable and do not differ in growth rate or plating efficiency from isogenic rad50-1 or Rad+ strains.
摘要
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