酿酒酵母中质粒DNA双链缺口修复的遗传控制
Genetic control of plasmid DNA double-strand gap repair in yeast, Saccharomyces cerevisiae.
作者信息
Glaser V M, Glasunov A V, Tevzadze G G, Perera J R, Shestakov S V
机构信息
Department of Genetics, Moscow State University, USSR.
出版信息
Curr Genet. 1990 Jul;18(1):1-5. doi: 10.1007/BF00321107.
The repair of double-strand gaps (DSGs) in the plasmid DNA of radiosensitive mutants of Saccharomyces cerevisiae has been analyzed. The proportion of repair events that resulted in complete plasmid DNA DSG recovery was close to 100% in Rad+ cells. Mutation rad55 does not influence the efficiency and preciseness of DSG repair. The mutant rad57, which is capable of recombinational DNA DSB repair, resulted in no DSG recovery. Mutation rad53 substantially inhibits the efficiency of DSG repair but does not influence the precision of repair. Plasmid DNA DSG repair is completely blocked by mutations rad50 and rad54.
对酿酒酵母辐射敏感突变体的质粒DNA中的双链缺口（DSG）修复进行了分析。在Rad+细胞中，导致质粒DNA DSG完全恢复的修复事件比例接近100%。rad55突变不影响DSG修复的效率和准确性。能够进行重组DNA DSB修复的rad57突变体未导致DSG恢复。rad53突变显著抑制DSG修复的效率，但不影响修复的准确性。rad50和rad54突变完全阻断了质粒DNA DSG修复。
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