Department of Chemical Engineering, Virginia Tech, Blacksburg, VA 24061, United States.
Department of Chemical Engineering, Virginia Tech, Blacksburg, VA 24061, United States.
Acta Biomater. 2021 Sep 15;132:421-436. doi: 10.1016/j.actbio.2020.11.044. Epub 2020 Dec 1.
Glioblastoma multiforme (GBM) is the most deadly form of brain cancer. Recurrence is common, and established therapies have not been able to significantly extend overall patient survival. One platform through which GBM research can progress is to design biomimetic systems for discovery and investigation into the mechanisms of invasion, cellular properties, as well as the efficacy of therapies. In this review, 2D and 3D GBM in vitro cultures will be discussed. We focus on the effects of biomaterial properties, interactions between stromal cells, and vascular influence on cancer cell survival and progression. This review will summarize critical findings in each of these areas and how they have led to a more comprehensive scientific understanding of GBM. STATEMENT OF SIGNIFICANCE: Glioblastoma multiforme (GBM) is the most deadly form of brain cancer. Recurrence is common, and established therapies have not been able to significantly extend overall patient survival. One platform through which GBM research can progress is to design biomimetic systems for discovery and investigation into the mechanisms of invasion, cellular properties, as well as the efficacy of therapies. In this review, 2D and 3D GBM in vitro cultures will be discussed. We focus on the effects of biomaterial properties, interactions between stromal cells and vascular influence on cancer cell survival and progression. This review will summarize critical findings in each of these areas and how they have lead to a more comprehensive scientific understanding of GBM.
多形性胶质母细胞瘤（GBM）是最致命的脑癌形式。复发很常见，已有的治疗方法未能显著延长患者的总生存期。GBM 研究进展的一个平台是设计仿生系统，以发现和研究侵袭、细胞特性以及治疗效果的机制。在这篇综述中，将讨论 2D 和 3DGBM 体外培养。我们重点介绍生物材料特性、基质细胞之间的相互作用以及血管对癌细胞存活和进展的影响。本综述将总结这些领域的关键发现，以及它们如何导致对 GBM 的更全面的科学理解。
