[菜籽饼中芥子碱的提取分离及黑色素生成抑制作用机制]
[Extraction and Separation of Sinapine from Rapeseed Cake and the Mode of Action of Melanin Production Inhibition].
作者信息
Yang Z T, Lu D X, Hong E-K, Zhang B Y, С Jiang M, Yang Y J, Zhang D J
机构信息
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016 P. R. China.
Research Center for High Altitude Medicine, Key Laboratory of Application and Foundation for High Altitude Medicine Research in Qinghai Province (Qinghai-Utah Joint Research Key Lab for High Altitude Medicine), Qinghai University, Xining, 810001 China.
出版信息
Mol Biol (Mosk). 2020 Nov-Dec;54(6):1037-1045. doi: 10.31857/S0026898420050122.
Brassica campestris L. is the important oil-bearing crop in China. Rapeseed cake is the main byproduct of rapeseed oil extraction. As the main active ingredient in rapeseed cake, sinapine has several important biological activities. Therefore, the inhibitory activity of sinapine on tyrosinase in vitro and its free radical-scavenging rate were determined. Tyrosinase activity in A-375 human melanocytes was also investigated and the effects of sinapine on the melanin content and its antioxidant effects on melanin biosynthesis were studied. The results showed that sinapine had significant antioxidant activity. Sinapine significantly inhibited A-375 human melanocytes in a dose-dependent manner. Sinapine inhibited melanin synthesis in A-375 cells by downregulating the mRNA and protein expression of TRP-1, TRP-2, and MITF factors. The results showed that rapeseed cake sinapine inhibited melanin production and could be used as a potential active ingredient in the development of whitening agents.
油菜是中国重要的油料作物。菜籽饼是菜籽油提取的主要副产品。作为菜籽饼中的主要活性成分，芥子碱具有多种重要的生物活性。因此，测定了芥子碱在体外对酪氨酸酶的抑制活性及其自由基清除率。还研究了芥子碱对A-375人黑素细胞中酪氨酸酶活性的影响，并研究了芥子碱对黑色素含量的影响及其对黑色素生物合成的抗氧化作用。结果表明，芥子碱具有显著的抗氧化活性。芥子碱以剂量依赖的方式显著抑制A-375人黑素细胞。芥子碱通过下调TRP-1、TRP-2和MITF因子的mRNA和蛋白表达来抑制A-375细胞中的黑色素合成。结果表明，菜籽饼芥子碱抑制黑色素生成，可作为美白剂开发中的潜在活性成分。
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