Laboratory of Biological Engineering, Department of Chemical and Food Engineering, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
Bioprocess and Metabolic Engineering Laboratory, School of Food Engineering, Department of Food Engineering, University of Campinas, Campinas, SP, Brazil.
Carbohydr Polym. 2021 Feb 1;253:117274. doi: 10.1016/j.carbpol.2020.117274. Epub 2020 Oct 21.
Hydrothermal processing is an interesting biorefinery technology for converting lignocellulosic biomass into biofuels and biocompounds. This process is based on the selective solubilization and depolymerization of hemicellulose fraction (xylan) and may be considered beneficial, due to the possibility of obtaining xylooligosaccharides (XOS) with a degree of polymerization (DP) suitable for prebiotic applications. This study evaluated the effect of pressure (2.5 and 10 MPa) in a kinetic study (30 min) of hydrothermal treatment (180 °C) to optimize the extraction of XOS from mango seed shell. Total reducing sugars (TRS) values were close to the maximum in 15 min showing a slower rate for both pressures after this time, but at 10 MPa the value was 20 % lower than at 2.5 MPa. Based on these results, a new extraction was performed at 2.5 MPa and 15 min, and the extracted XOS were quantified, yielding 393.44 mg XOS/g xylan. XOS with a degree of polymerization between X2-X6 corresponded to 82.24 mg/g and XOS with X > 6 (or soluble xylan) corresponded to 311.20 mg/g. A low amount of xylose (8.81 mg/g xylan) was released, resulting in a hemicellulose conversion of 40.2 %. In general, approximately 8.1 kg of total XOS was produced from 100 kg of dried mango seed shell (X2-X6-1.7 kg and X > 6-6.4 kg).
水热加工是一种将木质纤维素生物质转化为生物燃料和生物化合物的有趣的生物炼制技术。该过程基于半纤维素（木聚糖）的选择性溶解和解聚，并且由于可能获得聚合度（DP）适合益生元应用的木低聚糖（XOS），因此可能是有益的。本研究评估了压力（2.5 和 10 MPa）在水热处理（180°C）动力学研究（30 min）中的作用，以优化从芒果籽壳中提取 XOS。总还原糖（TRS）值在 15 分钟时接近最大值，此后两种压力下的速率均较慢，但在 10 MPa 时的值比在 2.5 MPa 时低 20％。基于这些结果，在 2.5 MPa 和 15 min 下进行了新的提取，并且对提取的 XOS 进行了定量，得到 393.44 mg XOS/g 木聚糖。聚合度在 X2-X6 之间的 XOS 对应于 82.24 mg/g，X>6（或可溶性木聚糖）的 XOS 对应于 311.20 mg/g。释放的木糖量很少（8.81 mg/g 木聚糖），导致半纤维素转化率为 40.2％。通常，从 100 kg 干芒果籽壳中可生产约 8.1 kg 的总 XOS（X2-X6-1.7 kg 和 X>6-6.4 kg）。
