Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Foundation, Hannover, Germany, Bischofsholer Damm 15, Hannover, D-30173, Germany.
Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Foundation, Hannover, Germany, Bischofsholer Damm 15, Hannover, D-30173, Germany.
Food Microbiol. 2021 Apr;94:103673. doi: 10.1016/j.fm.2020.103673. Epub 2020 Nov 3.
Ready-to-eat (RTE) sliced emulsion type sausages are sensitive to recontamination with Listeria (L.) monocytogenes during processing and packaging steps. Since Listeria spp. are able to grow on those products under cold storage conditions, taking steps to reduce the recontamination risk and implementing antibacterial hurdles contribute to consumer safety and increase the product quality. With this study data about the suitability of culture broth, cell-free supernatant (CFS) or concentrated bacteriocin preparations (CFS) of bacteriocin-producing lactic acid bacteria (LAB) obtained from fermented sausages from Germany as protective culture or antibacterial additive were provided. In different challenge tests, the potential of selected LAB or their preparations were investigated for their potential to reduce growth of L. monocytogenes and/or Brochothrix (B.) thermosphacta on sliced RTE emulsion type sausages under modified atmosphere or vacuum during refrigerated storage for a 21-day period. Applied LAB culture broth and CFS could not reduce the growth of L. monocytogenes or B. thermosphacta. On the other hand, samples treated with CFS obtained from Pediococcus spp. strains showed a significant inhibition (p < 0.05) of more than 1.5 log of the applied L. monocytogenes strains during the storage period. The growth of B. thermosphacta could not be influenced. Thereby, the need for concentrating preparations was shown to be important to obtain a suitable antibacterial preparation that would contribute to consumer safety and food quality when applied as a protective additive.
即食(RTE)切片乳化型香肠在加工和包装过程中容易受到李斯特菌(L.)单核细胞增生李斯特菌的再次污染。由于李斯特菌属能够在冷藏条件下在这些产品上生长,因此采取措施降低再次污染的风险并实施抗菌障碍有助于消费者安全并提高产品质量。本研究提供了来自德国发酵香肠的产细菌素乳酸菌(LAB)的培养肉汤、无细胞上清液(CFS)或浓缩细菌素制剂(CFS)的数据,这些数据适用于保护性培养或抗菌添加剂。在不同的挑战测试中,研究了选定的 LAB 或其制剂的潜在能力,以研究它们在冷藏期间 21 天内减少切片 RTE 乳化型香肠中李斯特菌和/或热死环丝菌(B.)生长的潜力,这些香肠处于改良气氛或真空下。应用的 LAB 培养肉汤和 CFS 不能减少李斯特菌或 B. thermosphacta 的生长。另一方面,用来自肠球菌属菌株的 CFS 处理的样品显示出对应用的李斯特菌菌株的生长有显著抑制(p < 0.05),超过 1.5 个对数级,在储存期间。B. thermosphacta 的生长不能受到影响。因此,需要浓缩制剂以获得合适的抗菌制剂,当用作保护性添加剂时,有助于消费者安全和食品质量。