Kerosuo E, Haapasalo M, Ranta H, Lounatmaa K
Department of Cariology, University of Helsinki, Finland.
J Gen Microbiol. 1987 Aug;133(8):2217-24. doi: 10.1099/00221287-133-8-2217.
In Bacteroides buccae, a hexagonally arranged periodic structure was found in the outer membrane (OM), in addition to hexagonal lattices present in its external surface layer (S-layer). This crystalline OM protein (COMP) was present as patches on the concave fracture face (the outer leaflet) of the OM in freeze-fractured cells. Occasionally, hexagonally arranged structures could also be seen on the convex fracture face of the OM as 'fingerprints' of the COMP. The OM proteins were isolated and analysed by gel electrophoresis. The major band protein had an apparent molecular mass of 17 kDa. Whether the minor band proteins are also components in the structure of the COMP remains to be elucidated. Other oral Gram-negative anaerobic rods studied did not show any periodicity in their OM.
在牙龈拟杆菌中，除了其外表面层（S层）存在六边形晶格外，在外膜（OM）中还发现了一种六边形排列的周期性结构。这种结晶性外膜蛋白（COMP）在冷冻断裂细胞的外膜凹面断裂面（外小叶）上呈斑块状存在。偶尔，在外膜的凸面断裂面上也可以看到六边形排列的结构，作为COMP的“指纹”。通过凝胶电泳分离并分析了外膜蛋白。主要条带蛋白的表观分子量为17 kDa。次要条带蛋白是否也是COMP结构的组成部分仍有待阐明。所研究的其他口腔革兰氏阴性厌氧杆菌在外膜中未显示任何周期性。
