Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai, Japan.
Graduate School of Life Sciences, Tohoku University, Sendai, Japan.
Methods Mol Biol. 2021;2346:51-62. doi: 10.1007/7651_2020_340.
The Drosophila melanogaster wing imaginal disc is an epithelial sac that exhibits dramatic tissue growth during the larval stage. With its simple morphology and accessibility of genetic tools, studies using the wing disc have contributed to the understanding of the mechanisms of epithelial homeostasis including the control of mitotic spindle orientation. This chapter describes a detailed protocol for analyzing epithelial architecture and planar orientation of the mitotic spindle in the wing disc epithelium. The rapid dissection method, effective immunostaining, and mounting tips described here facilitate genetic and cell biological studies of the wing disc and can be applied to a wide array of studies using various Drosophila tissues.
黑腹果蝇的翅原基是一个上皮囊，在幼虫阶段经历了显著的组织生长。由于其形态简单，且具有遗传工具的易获取性，利用翅原基进行的研究有助于理解上皮组织稳态的机制，包括有丝分裂纺锤体方向的控制。本章描述了一种详细的分析方案，用于研究翅原基上皮组织的形态和有丝分裂纺锤体的平面取向。这里描述的快速解剖方法、有效的免疫染色和装片方法有助于对翅原基进行遗传和细胞生物学研究，并且可以应用于各种使用不同 Drosophila 组织的研究中。
