Strassburger Katrin, Lorbeer Franziska K, Lutz Marilena, Graf Fabian, Boutros Michael, Teleman Aurelio A
German Cancer Research Center (DKFZ), Heidelberg 69120, Germany.
German Cancer Research Center (DKFZ), Heidelberg 69120, Germany
Development. 2017 Jul 1;144(13):2529-2538. doi: 10.1242/dev.147538. Epub 2017 May 19.
The wing imaginal disc has been an important model system over past decades for discovering novel biology related to development, signaling and epithelial morphogenesis. Novel experimental approaches have been enabled using a culture setup that allows cultures of wing discs. Current setups, however, are not able to sustain both growth and cell-cycle progression of wing discs We discover here a setup that requires both oxygenation of the tissue and adenosine deaminase activity in the medium, and supports both growth and proliferation of wing discs for 9 h. Nonetheless, further work will be required to extend the duration of the culturing and to enable live imaging of the cultured discs in the future.
在过去几十年里，翅成虫盘一直是发现与发育、信号传导和上皮形态发生相关新生物学的重要模型系统。利用一种允许培养翅成虫盘的培养装置，已经实现了新的实验方法。然而，目前的装置无法维持翅成虫盘的生长和细胞周期进程。我们在此发现了一种既需要组织氧合又需要培养基中腺苷脱氨酶活性的装置，它能支持翅成虫盘生长和增殖9小时。尽管如此，未来仍需要进一步开展工作来延长培养时间并实现对培养翅成虫盘的实时成像。
