College of Life and Health Sciences, Northeastern University, Shenyang, 110819, China.
Key Laboratory of Data Analytics and Optimization for Smart Industry, Northeastern University, Ministry of Education, Shenyang, 110819, China.
Mol Nutr Food Res. 2021 Jan;65(2):e2000711. doi: 10.1002/mnfr.202000711. Epub 2020 Dec 16.
In the present study, effect of pterostilbene on β-amyloid 1-42 (Aβ ) induced cognitive impairment in mice is investigated and explored its possible mechanism of action.
The behavior results show that pterostilbene alleviated Aβ -induces cognitive dysfunction assessed using the Y-maze test, novel object recognition task, Morris water maze test, and passive avoidance test. Pterostilbene alleviates neuron loss and accumulation of reactive oxygen species in Aβ treated mouse brain. Additionally, pterostilbene promotes nuclear factor-E2 p45-related factor 2 (Nrf2) nuclear translocation and enhance the transcription and expression of antioxidant genes such as heme oxygenase-1 and superoxide dismutase both in vivo and in vitro. Nrf2 inhibitor ML385 reverses the antioxidant function of pterostilbene in SH-SY5Y cells. Nrf2 is the master regulator of oxidative homeostasis and can be activated by substrate adaptor sequestosome-1 (also named p62). Pterostilbene promotes the binding of Kelch-like ECH-associated protein 1 and p62, which enhanced activation of Nrf2.
The present study reports that pterostilbene alleviated Aβ -induces cognitive dysfunction in mice. The mechanism of pterostilbene can be associated to the inhibition of oxidative stress through the Nrf2 signaling pathway.
在本研究中，研究了紫檀芪对β-淀粉样蛋白 1-42（Aβ）诱导的小鼠认知障碍的影响，并探讨了其可能的作用机制。
行为结果表明，紫檀芪减轻了 Y 迷宫测试、新物体识别任务、Morris 水迷宫测试和被动回避测试评估的 Aβ 诱导的认知功能障碍。紫檀芪减轻了 Aβ 处理的小鼠大脑中神经元丢失和活性氧的积累。此外，紫檀芪促进核因子-E2 p45 相关因子 2（Nrf2）核易位，并在体内和体外增强血红素加氧酶-1 和超氧化物歧化酶等抗氧化基因的转录和表达。Nrf2 抑制剂 ML385 逆转了紫檀芪在 SH-SY5Y 细胞中的抗氧化功能。Nrf2 是氧化平衡的主调节因子，可被底物衔接蛋白 sequestosome-1（也称为 p62）激活。紫檀芪促进 Kelch-like ECH-associated protein 1 和 p62 的结合，从而增强 Nrf2 的激活。
本研究报告称，紫檀芪减轻了 Aβ 诱导的小鼠认知功能障碍。紫檀芪的作用机制可能与通过 Nrf2 信号通路抑制氧化应激有关。
