Falode John Adeolu, Akinmoladun Afolabi Clement, Olaleye Mary Tolulope, Akindahunsi Afolabi Akintunde
Phytomedicine, Biochemical Pharmacology and Toxicology Unit, Department of Biochemistry, Federal University, Oye-Ekiti, Nigeria.
Phytomedicine, Biochemical Pharmacology and Toxicology Unit, Department of Biochemistry, School of Sciences, The Federal University of Technology, Akure, Nigeria.
Drug Chem Toxicol. 2022 Jul;45(4):1614-1624. doi: 10.1080/01480545.2020.1850757. Epub 2020 Dec 6.
The effect of on mitochondrial membrane permeability transition has not been explored. In this study, the effect of a solvent fraction of leaf extract on mitochondrial membrane permeability transition of rat brain and liver was evaluated. A methanol extract of leaves was fractionated into different solvents by vacuum liquid chromatography and following preliminary screening, the dichloromethane:ethylacetate (1:1) fraction was selected for further assays. Constituent phytochemicals in the fraction were revealed by thin-layer chromatography and further purification was carried out to characterize the compounds. Brain and liver mitochondria were isolated and used for mitochondrial membrane permeability transition and adenosine triphosphatase assays. Exogenous Ca and Al were used to trigger the mitochondrial membrane permeability transition opening. Physicochemical properties revealed by thin-layer chromatography showed that the isolated compounds were flavonoids. The extract inhibited mitochondrial membrane permeability transition opening in the presence and absence of triggering agents in brain and liver mitochondria. It also inhibited mitochondrial lipid peroxidation and adenosine triphosphatase activity. These results suggest that the extract can limit the rate of apoptosis via inhibition of mitochondrial membrane permeability transition which is pivotal to the mitochondrial apoptotic pathway and is an important therapeutic target in some pathological conditions.
关于[未提及具体物质]对线粒体膜通透性转换的影响尚未进行研究。在本研究中，评估了[未提及具体植物]叶提取物的一种溶剂级分对大鼠脑和肝线粒体膜通透性转换的影响。通过真空液相色谱法将[未提及具体植物]叶的甲醇提取物分离成不同溶剂，经过初步筛选后，选择二氯甲烷：乙酸乙酯（1:1）级分进行进一步测定。通过薄层色谱法揭示了该级分中的植物化学成分，并进行了进一步纯化以表征这些化合物。分离出脑和肝线粒体，并用于线粒体膜通透性转换和三磷酸腺苷酶测定。使用外源钙和铝来触发线粒体膜通透性转换孔的开放。薄层色谱法显示的物理化学性质表明分离出的化合物为黄酮类化合物。该提取物在有和没有触发剂的情况下均抑制脑和肝线粒体中的线粒体膜通透性转换孔开放。它还抑制线粒体脂质过氧化和三磷酸腺苷酶活性。这些结果表明，该提取物可通过抑制线粒体膜通透性转换来限制细胞凋亡速率，线粒体膜通透性转换对线粒体凋亡途径至关重要，并且在某些病理状况下是重要的治疗靶点。
