Laboratory of Neurobiology, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
Key Laboratory of Precision Nutrition and Food Quality, Ministry of Education, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
Stress. 2021 Sep;24(5):514-528. doi: 10.1080/10253890.2020.1855419. Epub 2020 Dec 15.
During pregnancy, uterus undergoes the environment adaptation as part of a program of development. In the world, one in four people worldwide suffer from mental illness, especially pregnant women. β-Adrenergic receptor (β-AR) is an important regulator that converts environmental stimuli into intracellular signals in mice uterus. CD-1 (ICR) mice undergone restraint stress, which was a case in model to simulate the psychological stress. The plasma and implantation sites in uterus were obtained and examined. PCR analysis demonstrated that β-AR expression levels in embryo day (E) 3, 5 and 7 were kept at a significantly higher level ( < 0.05) under restraint stress and higher than β-AR and β-AR in different gestation ages. The β-AR protein levels were obviously increased ( < 0.05) due to the markedly elevated norepinephrine (NE) concentration ( < 0.05). In our previous study, restraint stress can induce the apoptosis and inflammation. Also, the matrix metalloprotein-9 (MMP-9) was decreased significantly ( < 0.05) under restraint stress. Meanwhile, Caspase3, p-NF-κB p65 and p-ERK1/2 were obviously increased ( < 0.05) in the work. studies showed that the p-ERK1/2 and Caspase-3 levels were raised ( < 0.05) after β-AR was activated. However, they were decreased when PKA was blocked. The protein levels of Caspase-3 were reduced when ERK and NF-κB were blocked ( < 0.05). In conclusion, the β-AR/cAMP/PKA pathway promoted apoptosis and affected the development of the uterus through the ERK and NF-κB signaling pathway. The findings of this study may provide evidence for female reproduction under psychological stress.
在妊娠期间，子宫会经历环境适应，这是其发育计划的一部分。在世界范围内，全球有四分之一的人患有精神疾病，尤其是孕妇。β-肾上腺素能受体（β-AR）是一种重要的调节剂，可将环境刺激转化为小鼠子宫内的细胞内信号。CD-1（ICR）小鼠经历束缚应激，这是一种模拟心理应激的模型。获得并检查了血浆和子宫内的植入部位。PCR 分析表明，在束缚应激下，胚胎第 3、5 和 7 天的β-AR 表达水平保持在显著较高的水平（<0.05），并且高于β-AR 和不同妊娠年龄的β-AR。由于去甲肾上腺素（NE）浓度明显升高（<0.05），β-AR 蛋白水平明显升高（<0.05）。在我们之前的研究中，束缚应激会诱导细胞凋亡和炎症。此外，基质金属蛋白酶-9（MMP-9）在束缚应激下明显减少（<0.05）。同时，Caspase3、p-NF-κB p65 和 p-ERK1/2 在工作中明显增加（<0.05）。研究表明，β-AR 被激活后，p-ERK1/2 和 Caspase-3 水平升高（<0.05）。然而，当 PKA 被阻断时，它们会减少。ERK 和 NF-κB 被阻断时，Caspase-3 的蛋白水平降低（<0.05）。总之，β-AR/cAMP/PKA 途径通过 ERK 和 NF-κB 信号通路促进细胞凋亡并影响子宫发育。这项研究的结果可能为女性在心理应激下的生殖提供证据。
