水飞蓟宾与姜黄素联合使用降低MCF-7人乳腺癌细胞系中的瘦素受体表达。

Combination of Silibinin and Curcumin Reduced Leptin Receptor Expression in MCF-7 Human Breast Cancer Cell Line.

作者信息

Dalimi-Asl Somaye, Babaahmadi-Rezaei Hossein, Mohammadzadeh Ghorban

机构信息

Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Hyperlipidemia Research Center, Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

出版信息

Iran J Med Sci. 2020 Nov;45(6):477-484. doi: 10.30476/ijms.2019.81934.

DOI:10.30476/ijms.2019.81934

PMID:33281265

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7707627/

Abstract

BACKGROUND

Leptin and leptin receptor (Ob-R) are associated with worse prognosis, distant metastasis, and poor survival of breast cancer. We investigated the cytotoxic effect of silibinin and curcumin, individually and combined, on Ob-R expression in MCF-7 cells.

METHODS

This study was performed from October 2017 to April 2018 at the Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. The cytotoxic effect of silibinin and curcumin, individually and combined, and their corresponding half-maximal inhibitory concentration (IC) values were determined using the methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. The cells were treated with different concentrations of silibinin (50-400 μM), curcumin (10-35 μM), and their combinations for 24 and 48 hours. The expression of Ob-R was measured using the Western blot analysis by treating the cells with different concentrations of curcumin (10-25 μM), silibinin (50-250 μM), and their respective combinations. The difference in mean cell viability between the groups was calculated using one-way ANOVA followed by Tukey's test.

RESULTS

Silibinin and curcumin exerted time- and dose-dependent cytotoxic effect on MCF-7 cells. After treatment with silibinin, the IC values were about 250 and 50 μM at 24 and 48 hours, respectively. In terms of treatment with curcumin, the IC values were about 25 and 15 μM at 24 and 48 hours, respectively. Following treatment with silibinin, the Western blot analysis showed that Ob-R expression significantly decreased at 150 μM (P=0.031) and 200 μM (P=0.023) concentrations. Curcumin did not significantly decrease the Ob-R expression, however, the expression significantly decreased (P=0.004) when it was combined with silibinin.

CONCLUSION

The combination of silibinin and curcumin significantly reduced Ob-R expression in MCF-7 cells compared with their individual effects.

摘要

背景

瘦素和瘦素受体（Ob-R）与乳腺癌预后较差、远处转移及生存率低相关。我们研究了水飞蓟宾和姜黄素单独及联合使用对MCF-7细胞中Ob-R表达的细胞毒性作用。

方法

本研究于2017年10月至2018年4月在伊朗阿瓦士的阿瓦士军迪沙普尔医科大学医学院临床生物化学系进行。使用甲基噻唑基二苯基四氮唑溴盐（MTT）法测定水飞蓟宾和姜黄素单独及联合使用的细胞毒性作用及其相应的半数最大抑制浓度（IC）值。用不同浓度的水飞蓟宾（50 - 400μM）、姜黄素（10 - 35μM）及其组合处理细胞24小时和48小时。通过用不同浓度的姜黄素（10 - 25μM）、水飞蓟宾（50 - 250μM）及其各自组合处理细胞，使用蛋白质印迹分析测量Ob-R的表达。使用单因素方差分析和Tukey检验计算各组之间平均细胞活力的差异。

结果

水飞蓟宾和姜黄素对MCF-7细胞具有时间和剂量依赖性的细胞毒性作用。用水飞蓟宾处理后，24小时和48小时的IC值分别约为250μM和50μM。就用姜黄素处理而言，24小时和48小时的IC值分别约为25μM和15μM。用水飞蓟宾处理后，蛋白质印迹分析显示在150μM（P = 0.031）和200μM（P = 0.023）浓度下Ob-R表达显著降低。姜黄素未显著降低Ob-R表达，然而，当与水飞蓟宾联合使用时，其表达显著降低（P = 0.004）。