Nwaigwe Chioma U, Ihedioha John I, Shoyinka Shodeinde V, Nwaigwe Chukwuemeka O
Department of Veterinary Physiology and Pharmacology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria.
Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria.
Vet World. 2020 Oct;13(10):2294-2300. doi: 10.14202/vetworld.2020.2294-2300. Epub 2020 Oct 31.
Stress significantly affects health and productivity of animals. There is need for affordable and easy-to-assay markers of physiological stress in broilers. This study evaluated hematological and serum biochemical markers of physiologic stress in broiler chickens.
Thirty day-old broiler chickens were assigned to three groups of ten broilers each during the 56-day study. Group 1 broilers served as the control and were not exposed to any stressors; Group 2 broilers were stocked at the stress density of 50 kg BW m from day 49 to 56 while Group 3 broilers had their feed withdrawn 36 h before blood collection on day 56. Blood samples were collected on day 49 (pre-stress sample) and day 56 (post-stress sample) from all the birds and subjected to hematological and serum biochemical analysis.
The parameters did not vary significantly (p>0.05) pre-stress. Post-stress, there were significant variations in the heterophil, lymphocyte, and eosinophil counts and in the heterophil to lymphocyte ratio across the three groups. The concentrations of the plasma fibrinogen and serum albumins, sodium, and sodium to potassium ratio of the broilers varied significantly across the groups.
Heterophil, eosinophil, and lymphocyte counts, the heterophil to lymphocyte ratio, plasma fibrinogen, serum albumin, sodium, and sodium to potassium ratio are significant markers of physiological stress in broilers.
应激显著影响动物的健康和生产性能。肉鸡需要经济实惠且易于检测的生理应激标志物。本研究评估了肉鸡生理应激的血液学和血清生化标志物。
在为期56天的研究中，将30日龄的肉鸡分为三组，每组10只。第1组肉鸡作为对照，未暴露于任何应激源；第2组肉鸡从第49天至第56天以50 kg体重/平方米的应激密度饲养；第3组肉鸡在第56天采血前36小时禁食。在第49天（应激前样本）和第56天（应激后样本）采集所有鸡的血样，并进行血液学和血清生化分析。
应激前各参数差异不显著（p>0.05）。应激后，三组之间的嗜异性粒细胞、淋巴细胞和嗜酸性粒细胞计数以及嗜异性粒细胞与淋巴细胞比值存在显著差异。肉鸡的血浆纤维蛋白原、血清白蛋白、钠浓度以及钠钾比值在各组之间差异显著。
嗜异性粒细胞、嗜酸性粒细胞和淋巴细胞计数、嗜异性粒细胞与淋巴细胞比值、血浆纤维蛋白原、血清白蛋白、钠以及钠钾比值是肉鸡生理应激的重要标志物。
