Wu Wen-Hui, Shao Jian-Wei, Jiang Yong-Lin
Department of Laboratory Medicine, Yixing Traditional Chinese Medicine Hospital Wuxi 214200, Jiangsu Province, China,E-mial:
Department of Laboratory Medicine, Yixing People's Hospital, Wuxi 214200, Jiangsu Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Dec;28(6):1899-1903. doi: 10.19746/j.cnki.issn.1009-2137.2020.06.018.
To investigate the expression of costimulatory molecule Tim3 and its subsets on NK cells in patients with acute myeloid leukemia.
30 patients with acute myeloid leukemia treated in our hospital from August 2016 to August 2018 were randomly selected as the AML group, and 30 healthy persons in our hospital during the same period were randomly selected as the control group. The expression levels of CD56 NK cells/CD3CD56NK cells and Tim3 on CD56NK cells in peripheral blood, CD56 NK cells/CD3CD56NK cells and Tim3 on CD56NK cells in peripheral blood of the two groups were estimated. The expression of CD3CD56NK cells/lymphocytes and their Tim3, CD56NK cells/CD3CD56NK cells and their Tim3 expression, Tim3NK, Tim3NK IFN-gamma secretion levels before and after chemotherapy in the AML group were analyzed.
The expression levels of CD56 NK cells/CD3CD56NK cells and their Tim3 in peripheral blood of the AML group were significantly lower than those of the control group (P<0.05). The expression levels of CD56 NK cells/CD3CD56NK cells and their Tim3 in peripheral blood of the AML group were significantly higher than those of the control group (P<0.05). CD3CD56NK cells/lymphocytes and Tim3/CD3CD56NK cells in complete remission patients were significantly higher than at initial diagnosis (P<0.05). CD3CD56NK cells/lymphocytes and Tim3/CD3CD56NK cells in patients with course of treatment ≥2 times and remission patients time more than 3 months were significantly higher than those in patients with chemotherapy course 1 time and remission time≤3 months (P<0.05). The expression levels of CD56 NK cells/CD3CD56NK cells and their Tim3 in peripheral blood of patients at complete remission were significantly higher than at initial diagnosis (P<0.05). The expression levels of CD56 NK cells/CD3CD56NK cells and Tim3 in peripheral blood of patients at complete remission were significantly lower than of the patients at initial diagnosis (P<0.05), and the IFN-gamma secretion level of Tim3 NK cells was significantly lower than that of Tim3NK cells in these patients (P<0.05).
The expression of costimulatory molecule Tim3 in NK cells and its subsets of patients with acute myeloid leukemia is down-regulated.
探讨急性髓系白血病患者自然杀伤（NK）细胞共刺激分子Tim3及其亚群的表达情况。
随机选取2016年8月至2018年8月在我院治疗的30例急性髓系白血病患者作为急性髓系白血病（AML）组，同期随机选取我院30例健康者作为对照组。检测两组外周血中CD56 NK细胞/CD3CD56NK细胞及CD56NK细胞上Tim3的表达水平。分析AML组化疗前后CD3CD56NK细胞/淋巴细胞及其Tim3、CD56NK细胞/CD3CD56NK细胞及其Tim3表达、Tim3NK、Tim3NK IFN-γ分泌水平。
AML组外周血中CD56 NK细胞/CD3CD56NK细胞及其Tim3表达水平显著低于对照组（P<0.05）。AML组外周血中CD56 NK细胞/CD3CD56NK细胞及其Tim3表达水平显著高于对照组（P<0.05）。完全缓解患者的CD3CD56NK细胞/淋巴细胞及Tim3/CD3CD56NK细胞显著高于初诊时（P<0.05）。治疗疗程≥2次及缓解时间超过3个月患者的CD3CD56NK细胞/淋巴细胞及Tim3/CD3CD56NK细胞显著高于化疗疗程1次及缓解时间≤3个月患者（P<0.05）。完全缓解患者外周血中CD56 NK细胞/CD3CD56NK细胞及其Tim3表达水平显著高于初诊时（P<0.05）。完全缓解患者外周血中CD56 NK细胞/CD3CD56NK细胞及Tim3表达水平显著低于初诊患者（P<0.05），且这些患者中Tim3 NK细胞的IFN-γ分泌水平显著低于Tim3NK细胞（P<0.05）。
急性髓系白血病患者NK细胞及其亚群中共刺激分子Tim3的表达下调。
