[白细胞介素-27对过敏性紫癜患者Th17细胞的影响]

[Effects of IL-27 on Th17 Cells in Patients with Henoch-Schönlein Purpura].

作者信息

Li Ya-Feng, Xia Hai-Long

机构信息

Department of Hematology, The First Affiliated Hospital of Anhui Medical University，Hefei 230022, Anhui Province, China.

Department of Hematology, The First Affiliated Hospital of Anhui Medical University，Hefei 230022, Anhui Province, China,E-mail:

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Dec;28(6):2051-2055. doi: 10.19746/j.cnki.issn.1009-2137.2020.06.042.

DOI:10.19746/j.cnki.issn.1009-2137.2020.06.042

PMID:33283741

Abstract

OBJECTIVE

To investigate the effect of IL-27 on Th17 cells in patients with henoch-schönlein purpura（HSP） in order to further elucidate the pathogenesis.

METHODS

Fifty patients with HSP treated in our hospital from April 2019 to July 2019 were selected as HSP group, and 30 volunteers underwent physical examination at the same time were selected as control group. The proportion of Th17 cells in peripheral blood of HSP group and healthy control group was determined by flow cytometry (FCM). A total of 27 HSP patients were selected, and candidate peripheral blood mononuclear lymphocytes (PBMC) were co-cultured with exogenous rhIL-27, and the ratio of Th17 cells was detected by flow cytometry.

RESULTS

The proportion of Th17 cells in the peripheral blood of HSP patients with acute phase was (1.57±0.54)%, which was significantly higher than that of the control group (0.86±0.40)% (t=-6.298, P<0.001), and the proportion of Th17 cells was decreased significantly after rhIL-27 co-culture (1.39%±0.52% vs 0.98%±0.44%)(P<0.05).

CONCLUSION

IL-27 can reduce the level of Th17 cells in patients with HSP, which may be involved in the pathogenic process of HSP and play a protective role in the development of the disease.

摘要

目的

探讨白细胞介素-27（IL-27）对过敏性紫癜（HSP）患者Th17细胞的影响，以进一步阐明其发病机制。

方法

选取2019年4月至2019年7月在我院治疗的50例HSP患者作为HSP组，同时选取30例体检志愿者作为对照组。采用流式细胞术（FCM）检测HSP组和健康对照组外周血中Th17细胞的比例。选取27例HSP患者，将候选外周血单个核淋巴细胞（PBMC）与外源性重组人IL-27（rhIL-27）共培养，采用流式细胞术检测Th17细胞比例。