Liu Yan, Zhu Hai-Feng, Qiao Wen-Ben, Zhang Yi, Song Yong-Hong, Nie Xiang-Min, Zhu Chuan-Fu, Zhai Pei-Cong
HLA Laboratory, Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
HLA Laboratory, Blood Center of Shandong Province, Jinan 250014, Shandong Province, China,E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Dec;28(6):2066-2070. doi: 10.19746/j.cnki.issn.1009-2137.2020.06.044.
To study the Polymorphism of the human platelet antigen(HPA) gene 1-17 and human leukocyte antigen(HLA) gene-A and B locus in Shandong Han population.
A total of 962 samples from routine voluntary platelet donors were genotyped for HPA1-17 system and HLA-A site, B by PCR-SSP and PCR-SSOP respectively．Gene frequencies were calculated by counting. HPA1-17 and HLA genotype combinations were analyzed by Arelequin 3.5.
The gene frequencies of HPA-la, -1b, HPA-2a, -2b, HPA-3a, -3b, HPA-4a, -4b, HPA-5a, -5b, HPA-6a, -6b, HPA-15a, -15b were 0.9918, 0.0082, 0.9419, 0.0592, 0.5841, 0.4174, 0.9969, 0.0031, 0.9892, 0.0108, 0.9835, 0.0175,0.5488 and 0.4512, respectively． The most common HPA genotype combination was HPA-(1, 2, 4, 5, 6, 7-14, 16, 17) aa-3ab-15ab (0.2048). Moreover, HLA-A2(0.3094) and HLA-B13(0.1513) showed the highest frequency in their respective locus. The most common HLA genotype combination was HLA-A2-B13(0.1397) .
Distributions of HPA and HLA show high polymorphism in Shandong Han population. The ethnic and territorial difference of HPA distribution is also confirmed. It is imperative to establish local genetic database of volunteer platelet donors.
研究山东汉族人群人类血小板抗原（HPA）基因1 - 17以及人类白细胞抗原（HLA）基因A、B位点的多态性。
分别采用聚合酶链反应-序列特异性引物（PCR - SSP）和聚合酶链反应-序列特异性寡核苷酸探针（PCR - SSOP）技术，对962例常规自愿无偿献血者的样本进行HPA1 - 17系统及HLA - A位点、B位点基因分型。通过计数计算基因频率。利用Arlequin 3.5软件分析HPA1 - 17和HLA基因型组合。
HPA - 1a、- 1b、HPA - 2a、- 2b、HPA - 3a、- 3b、HPA - 4a、- 4b、HPA - 5a、- 5b、HPA - 6a、- 6b、HPA - 15a、- 15b的基因频率分别为0.9918、0.0082、0.9419、0.0592、0.5841、0.4174、0.9969、0.0031、0.9892、0.0108、0.9835、0.0175、0.5488和0.4512。最常见的HPA基因型组合为HPA - (1, 2, 4, 5, 6, 7 - 14, 16, 17) aa - 3ab - 15ab（0.2048）。此外，HLA - A2（0.3094）和HLA - B13（0.1513）在各自位点的频率最高。最常见的HLA基因型组合为HLA - A2 - B13（0.1397）。
山东汉族人群中HPA和HLA分布呈现高度多态性。同时也证实了HPA分布存在种族和地域差异。建立当地自愿无偿献血者基因数据库势在必行。
