Zhejiang Provincial Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis, Institute of Mass Spectrometry, Ningbo University, Ningbo 315211, P. R. China.
Analyst. 2021 Jan 4;146(1):75-84. doi: 10.1039/d0an01677b.
3-Pyridinylboronate, a zwitterionic boronic acid, displayed effective in situ ESI for reversible covalent tagging of saccharides in both cation and anion modes. The ion mobilities of thus-generated ions were examined with the Bruker timsTOF fleX instrument. Nine disaccharides were examined using this method. They have identical mass-to-charge ratios, differing only in monomer compositions, regio-linkages, and anomeric configurations (α or β). The IMS separations of the disaccharides from this method were compared with those from sodium adducts reported in the literature. The differentiation effects of this method on the disaccharide isomers were increased on average by an order of magnitude. Using this method, all the pairs of disaccharides selected from nine isomers were completely identified by comparing the mobility spectra of single-tagged and double-tagged ions.
3-吡啶硼酸酯，一种两性离子硼酸，在阳离子和阴离子模式下均能有效地进行可逆的糖的共价标记。利用 Bruker timsTOF fleX 仪器对生成的离子的离子迁移率进行了检测。用该方法检测了 9 种二糖。它们的质荷比相同，仅在单体组成、区域连接和端基构型（α 或β）上有所不同。与文献中报道的钠加合物相比，该方法对二糖的 IMS 分离效果提高了一个数量级。利用该方法，通过比较单标记和双标记离子的迁移谱，从 9 种异构体中选择的所有二糖对都能被完全识别。
